Anti-Grp75/HSPA9 Antibody Picoband™

GRP75/HSPA9B/Mortalin antibody

Boster Bio Anti-Grp75/HSPA9 Antibody Picoband™ catalog # PB9642. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9642
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Grp75/HSPA9 Antibody Picoband™

View all GRP75/HSPA9B/Mortalin Antibodies

SKU/Catalog Number

PB9642

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Grp75/HSPA9 Antibody Picoband™ catalog # PB9642. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Grp75/HSPA9 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9642)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Grp75, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9642 is reactive to HSPA9 in Human, Mouse, Rat

Applications

PB9642 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

73.68kDa

Background of GRP75/HSPA9B/Mortalin

HSPA9 (heat shock 70kDa protein 9 (mortalin)), also known as GRP75, mot-2, mthsp75, PBP74, HSPA9B, MORTALIN or MORTALIN, PERINUCLEAR, is a highly conserved member of the HSP70 family of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome. The HSPA9 gene is mapped to chromosome 5q31.2 based on an alignment of the HSPA9 sequence with the genomic sequence. Knockdown of HSPA9 in erythroid cultures was associated with an increased number of cells in the G0/G1 phase of the cell cycle and accelerated apoptosis. Knockdown of Hspa9 in mouse bone marrow cells, followed by transplantation into wildtype recipients, also resulted in loss of erythroid cell number. Haploinsufficiency for HSPA9 may contribute to abnormal hematopoiesis in myelodysplastic syndromes. This protein plays a role in the control of cell proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSPA9 (Source: Uniprot.org, NCBI)

Gene Name

HSPA9

Full Name

Stress-70 protein, mitochondrial

Weight

73.68kDa

Superfamily

heat shock protein 70 family

Alternative Names

75 kDa glucose-regulated protein; CSA; GRP75; GRP-75; GRP75mthsp75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9B (mortalin-2); HSPA9; HSPA9B; HSPA9BMGC4500; Mortalin; mortalin, perinuclear; Mortalin-2; MOT; MOT2; mot-2; MTHSP75; p66-mortalin; PBP74; PBP74CSA; Peptide-binding protein 74; stress-70 protein, mitochondrial HSPA9 CRP40, CSA, EVPLS, GRP-75, GRP75, HEL-S-124mB, MOT, MOT2, MTHSP75, PBP74, SAAN, SIDBA4, HSPA9 heat shock protein family A (Hsp70) member 9 stress-70 protein, mitochondrial|75 kDa glucose-regulated protein|catecholamine-regulated protein 40|epididymis secretory sperm binding protein Li 124m|heat shock 70kD protein 9B|heat shock 70kDa protein 9 (mortalin)|mortalin, perinuclear|mortalin-2|mortalin2|p66-mortalin|peptide-binding protein 74

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPA9, check out the HSPA9 Infographic

HSPA9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPA9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-Grp75/HSPA9 Antibody Picoband™

Question

We are currently using anti-Grp75/HSPA9 antibody PB9642 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

The anti-Grp75/HSPA9 antibody (PB9642) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-11-22

Order DetailsPrice
PB9642

100μg

$370
PB9642-10ug

10μg sample (liquid)

$99
PB9642-Biotin

100 μg Biotin conjugated

$570
PB9642-Cy3

100 μg Cy3 conjugated

$570
PB9642-Dylight488

100 μg Dylight488 conjugated

$570
PB9642-Dylight550

100 μg Dylight550 conjugated

$570
PB9642-Dylight594

100 μg Dylight594 conjugated

$570
PB9642-FITC

100 μg FITC conjugated

$570
PB9642-HRP

100 μg HRP conjugated

$570
PB9642-APC

100 μg APC conjugated

$670
PB9642-PE

100 μg PE conjugated

$670
PB9642-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9642
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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