|Application:||IHC-P, IHC-F, ICC, WB|
Data & Images
|Product Name||Anti-GRP94 Antibody|
|Description||Rabbit IgG polyclonal antibody for Endoplasmin(HSP90B1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-GRP94 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1340)|
|Replacement Item||This antibody may replace the following items: sc-1055|sc-11402|sc-13119|sc-13119-X|sc-1794|sc-32249|sc-393402|sc-53929|sc-56399|sc-59577|sc-59578|sc-7947|sc-8262 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human GRP94(687-700aa INPRHPLIRDMLRR), identical to the related mouse and rat sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Gene Full Name||Endoplasmin|
|Protein Function||Molecular chaperone that functions in the processing andtransport of secreted proteins. When associated with CNPY3,required for proper folding of Toll-like receptors (Bysimilarity). Functions in endoplasmic reticulum associateddegradation (ERAD). Has ATPase activity..|
|Subcellular Localization||Endoplasmic reticulum lumen. Melanosome.Identified by mass spectrometry in melanosome fractions fromstage I to stage IV.|
|Uniprot ID||HSP90B1: P14625
|Alternative Names||Endoplasmin; 94 kDa glucose-regulated protein; GRP-94; Heat shock protein 90 kDa beta member 1; Tumor rejection antigen 1; gp96 homolog; HSP90B1; GRP94, TRA1;|
|Research Areas||tags & cell markers|subcellular markers|organelles|er|signal transduction|protein trafficking|er proteins|cancer|cancer metabolism|response to hypoxia|pathways and processes|metabolism processes|types of disease||
Background for Endoplasmin
At Boster we strive to provide the best Anti-GRP94 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
|Immunohistochemistry(Frozen Section)||0.5-1μg/ml||Rat||Human, Mouse|
|Immunohistochemistry(Paraffin-embedded Section)||0.5-1μg/ml||Human, Mouse, Rat||-||By Heat|
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GRP94 Antibody Images
Click the images to enlarge.
Lane 1: RAJI Cell Lysate
Lane 2: MCF-7 Cell Lysate
IHC(P): HELA Cell
IHC(P): Human Lung Cancer Tissue
IHC(P): Rat Brain Tissue
- Review by R.P.
"Reliable and robust"
--R.P., BIOLOGY, PENN STATE UNIVERSITY, TECHNICIAN
Applications Western Blot Sample Cell lysate Detection Enhanced chemiluminescence
"This is an excellent antibody to endoplasmin in HC11 cells. Clean, reliable detection with very little if any background. Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,