SKU M00791
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number EB-7
Host Rabbit
Applications IF, IHC, WB

Overview

Product Name Anti-GSK3 beta Rabbit Monoclonal Antibody
SKU/Catalog Number M00791
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-GSK3 beta Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, IHC, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-GSK3 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00791)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human GSK3 beta
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500-1:3000
IHC 1:50-1:200
ICC/IF 1:100-1:500
Flow Cytometry 1:200-1:500

Images And Assay Conditions

/M/0/M00791 GSK3B primary antibodies IHC testing 1.jpg

Immunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody(M00791)
GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (M00791)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/M/0/M00791 GSK3B primary antibodies WB testing 1.jpg

Western blot analysis of GSK3 beta expression in HEK293 cell lysate (M00791).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3B monoclonal antibody (Catalog # M00791) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3B

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P49841
Gene Name GSK3B
Protein Name Glycogen synthase kinase-3 beta
Tissue Specificity Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
Alternative Names Glycogen synthase kinase-3 beta;GSK-3 beta;2.7.11.26;Xgsk-3 protein;gsk3b;Xgsk-3;
Subcellular Localization Cytoplasm . Nucleus . Cell membrane .
Molecular Weight 46903 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Plays a role in the organization of the formation of the main body axis of developing embryo. Acts as an inhibitor of differentiation of primary neurons. Inhibits the ability of ectopically expressed NEUROD1 and other bHLH factors to promote early retinal cell differentiation. May participate in the Wnt signaling pathway. May regulate the circadian clock via phosphorylation of the major clock components. .
Research Areas Carbohydrate Metabolism, Cell Biology, Cell Cycle, Cell Differentiation, Developmental Biology, Energy Metabolism, Metabolic Signaling Pathways, Metabolism, Nervous System Development, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Pathways And Processes, Signal Transduction

*You can search these to find other products in these research areas.

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Order Product (M00791)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

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