|Product Name||Anti-GSK3 beta Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-GSK3 beta Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, IHC, ICC, WB in Human, Mouse|
|Cite This Product||Anti-GSK3 beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00791)|
|Immunogen||A synthesized peptide derived from human GSK3 beta|
Assay Dilutions Overview
Flow Cytometry 1:200-1:500
Images And Assay Conditions
Immunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody(M00791)
GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (M00791)overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Western blot analysis of GSK3 beta expression in HEK293 cell lysate (M00791).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3B monoclonal antibody (Catalog # M00791) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3B
Protein Target Info (Source: Uniprot.org)
|Protein Name||Glycogen synthase kinase-3 beta|
|Tissue Specificity||Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.|
|Alternative Names||Glycogen synthase kinase-3 beta;GSK-3 beta;220.127.116.11;Xgsk-3 protein;gsk3b;Xgsk-3;|
|Subcellular Localization||Cytoplasm . Nucleus . Cell membrane .|
|Molecular Weight||46903 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Plays a role in the organization of the formation of the main body axis of developing embryo. Acts as an inhibitor of differentiation of primary neurons. Inhibits the ability of ectopically expressed NEUROD1 and other bHLH factors to promote early retinal cell differentiation. May participate in the Wnt signaling pathway. May regulate the circadian clock via phosphorylation of the major clock components. .|
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