Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated

SKU HT0015
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number DH-7
Host Rabbit
Applications WB


Product Name Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated
SKU/Catalog Number HT0015
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated tested for WB in Human, Mouse, Rat
Cite This Product Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # HT0015)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from GST
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:3000-1:10000

Images And Assay Conditions

/H/T/HT0015 GST Tag primary antibodies WB testing 1.jpg

Western blot analysis of GST expression in GST recombinant protein, using GST tag Antibody(HRP conjugated) (HT0015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST-Tag monoclonal antibody (Catalog # HT0015) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST-Tag

Target Info

Protein Target Info (Source:

Gene Name GST-Tag

*if product is indicated to react with multiple species, protein info is based on the human gene.


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Order Product (HT0015)


Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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