Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated

SKU HT0015
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number DH-7
Host Rabbit
Applications WB

Overview

Product Name Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated
SKU/Catalog Number HT0015
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated tested for WB in Human, Mouse, Rat
Cite This Product Anti-GST-Tag Rabbit Monoclonal Antibody, HRP Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # HT0015)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from GST
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:3000-1:10000

Images And Assay Conditions

/H/T/HT0015 GST Tag primary antibodies WB testing 1.jpg

Western blot analysis of GST expression in GST recombinant protein, using GST tag Antibody(HRP conjugated) (HT0015).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST-Tag monoclonal antibody (Catalog # HT0015) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST-Tag

Target Info

Protein Target Info (Source: Uniprot.org)

Gene Name GST-Tag

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Order Product (HT0015)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

Ships in 7-10 business days.

Troubleshooting

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

We promise all of our products perform as described in datasheets.

Write a review for HT0015