Data & Images
|Product Name||Anti-GST3/GST Pi Antibody|
|Description||Rabbit IgG polyclonal antibody for Glutathione S-transferase P(GSTP1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-GST3/GST Pi Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1590)|
|Replacement Item||This antibody may replace the following items: sc-134469|sc-28494|sc-376013|sc-376481|sc-66000 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human GST3/GST pi(12-32aa RGRCAALRMLLADQGQSWKEE), different from the related rat and mouse sequences by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Glutathione S-transferase P|
|Molecular Weight||23356 MW|
|Protein Function||Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. .|
|Sequence Similarities||Belongs to the GST superfamily. Pi family.|
|Subcellular Localization||Cytoplasm . Mitochondrion . Nucleus . The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.|
|Alternative Names||Glutathione S-transferase P;126.96.36.199;GST class-pi;GSTP1-1;GSTP1;FAEES3, GST3;|
|Research Areas|||cardiovascular|blood| neuroscience|neurology process|metabolism|neurogenesis| metabolism|pathways and processes|mitochondrial metabolism|mitochondrial markers|redox metabolism|oxidative stress|types of disease|cancer||
Background for Glutathione S-transferase P
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-GST3/GST Pi Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GST3/GST Pi Antibody Images
Click the images to enlarge.
IHC(P): Human Intestinal Cancer Tissue
All lanes: Anti GST3/GST pi (PA1590) at 0.5ug/ml
Lane 1: Rat Kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 23KD
Observed bind size: 23KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,