Data & Images
|Product Name||Anti-Haptoglobin Antibody|
|Description||Rabbit IgG polyclonal antibody for Haptoglobin(HP) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Haptoglobin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1599)|
|Replacement Item||This antibody may replace the following items: sc-134464|sc-134465|sc-134466|sc-19634|sc-22915|sc-22916|sc-25871|sc-271915|sc-28434|sc-292875|sc-32931|sc-365396|sc-374208|sc-376893|sc-390796|sc-390962|sc-50344|sc-514057|sc-51625|sc-55957-R|sc-55959-R|sc-59143|sc-65270|sc-68667|sc-68668|sc-69782|sc-69783|sc-69891|sc-69892 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Haptoglobin(294-309aa DHLKYVMLPVADQDQC).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||45205 MW|
|Protein Function||As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway. .|
|Tissue Specificity||Expressed by the liver and secreted in plasma.|
|Sequence Similarities||Belongs to the peptidase S1 family.|
|Alternative Names||Haptoglobin;Zonulin;Haptoglobin alpha chain;Haptoglobin beta chain;HP;|
|Research Areas|||tags & cell markers|epitope tags|conjugates||
Background for Haptoglobin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Haptoglobin Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Haptoglobin Antibody Images
Click the images to enlarge.
Lane 1:RAJI Cell Lysate
Lane 2:HL-60 Cell Lysate
Lane 3:HUT102 Cell Lysate
Lane 4:JURKAT Cell Lysate
Lane 5:CEM Cell Lysate
IHC(P): Human Lung Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,