Anti-HBG1/2 Rabbit Monoclonal Antibody

HBG1 antibody

Boster Bio Anti-HBG1/2 Rabbit Monoclonal Antibody catalog # M02830. Tested in WB application. This antibody reacts with Human.

Product Info Summary

SKU: M02830
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-HBG1/2 Rabbit Monoclonal Antibody

View all HBG1 Antibodies

SKU/Catalog Number

M02830

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-HBG1/2 Rabbit Monoclonal Antibody catalog # M02830. Tested in WB application. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HBG1/2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02830)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AADB-8

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human HBG1/2

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M02830 is reactive to HBG1 in Human

Applications

M02830 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

30 kDa

Calculated molecular weight

16.14kDa

Background of HBG1

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For HBG1 (Source: Uniprot.org, NCBI)

Gene Name

HBG1

Full Name

Hemoglobin subunit gamma-1

Weight

16.14kDa

Superfamily

globin family

Alternative Names

A-gamma globin; gamma A hemoglobin; gamma globin; gamma-1-globin; Hb F Agamma; HBGA; HBGR; Hemoglobin gamma-1 chain; Hemoglobin gamma-A chain; hemoglobin subunit gamma-1; hemoglobin, gamma A; hemoglobin, gamma, regulator of; HSGGL1 HBG1 HBG-T2, HBGA, HBGR, HSGGL1, PRO2979 hemoglobin subunit gamma 1 hemoglobin subunit gamma-1|A-gamma globin|gamma A hemoglobin|gamma globin|gamma-1-globin|hb F Agamma|hemoglobin gamma-1 chain|hemoglobin gamma-a chain|hemoglobin, gamma A|hemoglobin, gamma, regulator of

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HBG1, check out the HBG1 Infographic

HBG1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HBG1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-HBG1/2 Rabbit Monoclonal Antibody

Question

I was wanting to use your anti-HBG1/2 Rabbit Monoclonal antibody for WB for human fetal liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fetal liver identification?

Verified Customer

Verified customer

Asked: 2019-10-02

Answer

It shows on the product datasheet, M02830 anti-HBG1/2 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human fetal liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-02

Question

We are currently using anti-HBG1/2 Rabbit Monoclonal antibody M02830 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-30

Answer

The anti-HBG1/2 Rabbit Monoclonal antibody (M02830) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-30

Question

I have attached the WB image, lot number and protocol we used for fetal liver using anti-HBG1/2 Rabbit Monoclonal antibody M02830. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-06-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-06-30

Question

Does M02830 anti-HBG1/2 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Baker

Verified customer

Asked: 2014-02-05

Answer

As indicated on the product datasheet, M02830 anti-HBG1/2 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-02-05

Order DetailsPrice
M02830

100μL

$370
M02830-30ul

30μL sample

$99
M02830-carrier-free

Carrier Free

$370

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M02830
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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