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SKU:PA1552
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-hCG Receptor Antibody
SKU/Catalog Number PA1552
Description Rabbit IgG polyclonal antibody for Lutropin-choriogonadotropic hormone receptor(LHCGR) detection. Tested with WB, IHC-P in Human;Rat.
Cite This Product Anti-hCG Receptor Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1552)
Replacement Item This antibody may replace the following items: sc-25828|sc-26341|sc-26342 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Predicted Species Canine, Horse, Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human hCG receptor(127-143aa YLSICNTGIRKFPDVTK), different from the relative rat sequence by two amino acids, mouse sequence by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name LHCGR
Protein Name Lutropin-choriogonadotropic hormone receptor(LH/CG-R)
Molecular Weight 78643 MW
Protein Function Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.
Tissue Specificity Gonadal and thyroid cells.
Sequence Similarities Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.
Subcellular Localization Cell membrane; Multi-pass membrane protein.
Uniprot ID P22888
Alternative Names Lutropin-choriogonadotropic hormone receptor;LH/CG-R;Luteinizing hormone receptor;LHR;LSH-R;LHCGR;LCGR, LGR2, LHRHR;
Research Areas |neuroscience|neurotransmission|receptors / channels|gpcr|more gpcr| signal transduction|signaling pathway|g protein signaling| neuroscience|endocrine system|gonadotrophic axis|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Lutropin-choriogonadotropic hormone receptor(LH/CG-R)

The luteinizing hormone/choriogonadotropin receptor is a member of a subfamily of G protein-coupled receptors(GPCR) characterized by the presence of a large N-terminal extracellular domain containing several leucine-rich repeats(LRR). This glycoprotein hormone receptor family has been named the LRR-containing GPCR(LGR) family. The LHCGR gene contains 11 exons and spans approximately 80 kb and it is mapped to chromosome 2p21. In the ovary, theca, stromal, late-stage(luteinizing) granulosa, and luteal cells contain LHCGR. In the testes, Leydig cells contain LHCRG.

Anti-hCG Receptor Antibody Images

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Anti-hCG Receptor Antibody
Anti-hCG receptor antibody, PA1552, IHC(P)
IHC(P): Rat Ovary Tissue
Anti-hCG Receptor Antibody
Anti-hCG receptor antibody, PA1552, Western blotting
All lanes: Anti hCG receptor (PA1552) at 0.5ug/ml
WB: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: What are your suggestions to reduce the background for my western blot? Keyword: troubleshooting, protocol support
A: Our technicians provide these suggestions to reduce the background: 1.Block the membrane 2 hours at room temperature with 5% no-fat milk in TBS-T; 2.Dilute second antibody with 5% no-fat milk in TBS-T; and 3.Extend the wash time to 10 minutes each time.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.