Anti-HCN1 Antibody Picoband™

Hcn1 antibody

Boster Bio Anti-HCN1 Antibody Picoband™ catalog # PB9210. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9210
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-HCN1 Antibody Picoband™

View all Hcn1 Antibodies

SKU/Catalog Number

PB9210

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HCN1 Antibody Picoband™ catalog # PB9210. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HCN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9210)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9210 is reactive to Hcn1 in Human, Mouse, Rat

Applications

PB9210 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

102.432kDa

Background of Hcn1

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, also known as HAC-2 or BCNG-1, is a protein that in humans is encoded by the HCN1 gene. It is mapped to 5p12. The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes. Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. It may mediates responses to sour stimuli.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Hcn1 (Source: Uniprot.org, NCBI)

Gene Name

Hcn1

Full Name

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Weight

102.432kDa

Superfamily

potassium channel HCN family

Alternative Names

BCNG1; BCNG-1brain cyclic nucleotide gated channel 1; Brain cyclic nucleotide-gated channel 1; HAC-2; hyperpolarization activated cyclic nucleotide-gated potassium channel 1; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 Hcn1|Bcng, Bcng1, C630013B14Rik, HAC, HAC2|hyperpolarization activated cyclic nucleotide gated potassium channel 1|potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1|BCNG-1|HAC-2|brain cyclic nucleotide-gated channel 1|hyperpolarization-activated cation channel 2|hyperpolarization-activated, cyclic nucleotide-gated K+ 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Hcn1, check out the Hcn1 Infographic

Hcn1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Hcn1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-HCN1 Antibody Picoband™

Question

I see that the anti-HCN1 antibody PB9210 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-17

Question

Does PB9210 anti-HCN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

You can see on the product datasheet, PB9210 anti-HCN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-31

Question

I was wanting to use your anti-HCN1 antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

It shows on the product datasheet, PB9210 anti-HCN1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-24

Question

Is this PB9210 anti-HCN1 antibody reactive to the isotypes of HCN1?

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

The immunogen of PB9210 anti-HCN1 antibody is E.coli-derived human HCN1 recombinant protein (Position: E618-L890). Human HCN1 shares 82% amino acid (aa) sequence identity with both mouse and rat HCN1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-26

Question

We need to test anti-HCN1 antibody PB9210 on rat brain for research purposes, then I may be interested in using anti-HCN1 antibody PB9210 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Thomas

Verified customer

Asked: 2019-08-08

Answer

The products we sell, including anti-HCN1 antibody PB9210, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-08-08

Question

Is a blocking peptide available for product anti-HCN1 antibody (PB9210)?

Verified Customer

Verified customer

Asked: 2019-06-07

Answer

We do provide the blocking peptide for product anti-HCN1 antibody (PB9210). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-07

Question

We are currently using anti-HCN1 antibody PB9210 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

The anti-HCN1 antibody (PB9210) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-29

Order DetailsPrice
PB9210

100μg

$370
PB9210-10ug

10μg sample (liquid)

$99
PB9210-Biotin

100 μg Biotin conjugated

$570
PB9210-Cy3

100 μg Cy3 conjugated

$570
PB9210-Dylight488

100 μg Dylight488 conjugated

$570
PB9210-Dylight550

100 μg Dylight550 conjugated

$570
PB9210-Dylight594

100 μg Dylight594 conjugated

$570
PB9210-FITC

100 μg FITC conjugated

$570
PB9210-HRP

100 μg HRP conjugated

$570
PB9210-APC

100 μg APC conjugated

$670
PB9210-PE

100 μg PE conjugated

$670
PB9210-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PB9210
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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