Data & Images
|Product Name||Anti-HDAC2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Histone deacetylase 2(HDAC2) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-HDAC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1350)|
|Replacement Item||This antibody may replace the following items: sc-56685|sc-7899|sc-55542|sc-9959|sc-6296|sc-55541|sc-81599|sc-6296-R|sc-7899-X|sc-6296-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human HDAC2(464-478aa EDKSKDNSGEKTDTK), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Histone deacetylase 2(HD2)|
|Molecular Weight||55364 MW|
|Protein Function||Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. May be involved in the transcriptional repression of circadian target genes, such as PER1, mediated by CRY1 through histone deacetylation. Involved in MTA1-mediated transcriptional corepression of TFF1 and CDKN1A. .|
|Tissue Specificity||Widely expressed; lower levels in brain and lung.|
|Sequence Similarities||Belongs to the histone deacetylase family. HD type 1 subfamily.|
|Subcellular Localization||Nucleus . Cytoplasm .|
|Alternative Names||Histone deacetylase 2;HD2;184.108.40.206;HDAC2;|
|Research Areas|||epigenetics and nuclear signaling|chromatin modifying enzymes|acetylation| stem cells|signaling pathways|wnt|hdacs| epigenetics and nuclear signaling|class i| cardiovascular|heart|hypertrophy||
Background for Histone deacetylase 2(HD2)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HDAC2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HDAC2 Antibody Images
Click the images to enlarge.
Lane 1: MM453 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: SMMC Cell Lysate
Lane 5: COLO320 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,