Anti-Her3/ErbB3 (Ab-1328) Antibody

ErbB3/Her3 antibody

Boster Bio Anti-Her3/ErbB3 (Ab-1328) Antibody catalog # A00539-2. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Product Info Summary

SKU: A00539-2
Size: 100ul
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Her3/ErbB3 (Ab-1328) Antibody

View all ErbB3/Her3 Antibodies

SKU/Catalog Number

A00539-2

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Her3/ErbB3 (Ab-1328) Antibody catalog # A00539-2. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Her3/ErbB3 (Ab-1328) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00539-2)

Host

Rabbit

Contents

Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

Peptide sequence around aa. 1326~1330 (P-D-Y-W-H) derived from Human Her3/ErbB3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00539-2 is reactive to ERBB3 in Mouse, Rat

Applications

A00539-2 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Predicted MW: 185kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100

Validation Images & Assay Conditions

Gene/Protein Information For ERBB3 (Source: Uniprot.org, NCBI)

Uniprot ID

P21860

Gene ID

2065

Gene Name

ERBB3

Full Name

Receptor tyrosine-protein kinase erbB-3

Weight

148.098kDa

Superfamily

protein kinase superfamily

Alternative Names

c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB3; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) ERBB3 ErbB-3, FERLK, HER3, LCCS2, MDA-BF-1, c-erbB-3, c-erbB3, erbB3-S, p180-ErbB3, p45-sErbB3, p85-sErbB3 erb-b2 receptor tyrosine kinase 3 receptor tyrosine-protein kinase erbB-3|human epidermal growth factor receptor 3|proto-oncogene-like protein c-ErbB-3|tyrosine kinase-type cell surface receptor HER3|v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ERBB3, check out the ERBB3 Infographic

ERBB3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ERBB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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15 Customer Q&As for Anti-Her3/ErbB3 (Ab-1328) Antibody

Question

Will A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Z. Jackson

Verified customer

Asked: 2020-03-27

Answer

It shows on the product datasheet, A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-27

Question

Would anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 work for WB with liver?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

According to the expression profile of liver, ERBB3 is highly expressed in liver. So, it is likely that anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 will work for WB with liver.

Boster Scientific Support

Answered: 2020-03-23

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-19

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-19

Question

We have been able to see staining in rat placenta small intestine. Any tips? Is anti-Her3/ErbB3 (Ab-1328) antibody supposed to stain placenta small intestine positively?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

According to literature placenta small intestine does express ERBB3. According to Uniprot.org, ERBB3 is expressed in corpus callosum, placenta, hippocampus, placenta small intestine, skin, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2020-03-05

Question

Our lab used your anti-Her3/ErbB3 (Ab-1328) antibody for WB on skin in the past. I am using mouse, and I plan to use the antibody for IHC next. I am looking for examining skin as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

M. Singh

Verified customer

Asked: 2020-02-14

Answer

I took a look at the website and datasheets of our anti-Her3/ErbB3 (Ab-1328) antibody and it seems that A00539-2 has been validated on mouse in both WB and IHC. Thus A00539-2 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-14

Question

See below the WB image, lot number and protocol we used for liver using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2. Please let me know if you require anything else.

D. Krishna

Verified customer

Asked: 2019-11-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-11-21

Question

I see that the anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-11

Question

Do you have a BSA free version of anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 available?

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-26

Question

We are currently using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

The anti-Her3/ErbB3 (Ab-1328) antibody (A00539-2) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-02

Question

I have a question about product A00539-2, anti-Her3/ErbB3 (Ab-1328) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-07

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-07

Question

I was wanting to use your anti-Her3/ErbB3 (Ab-1328) antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?

J. Collins

Verified customer

Asked: 2018-11-01

Answer

As indicated on the product datasheet, A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-01

Question

Is a blocking peptide available for product anti-Her3/ErbB3 (Ab-1328) antibody (A00539-2)?

Verified Customer

Verified customer

Asked: 2018-08-03

Answer

We do provide the blocking peptide for product anti-Her3/ErbB3 (Ab-1328) antibody (A00539-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-08-03

Question

We want to test anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 on mouse liver for research purposes, then I may be interested in using anti-Her3/ErbB3 (Ab-1328) antibody A00539-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-01-29

Answer

The products we sell, including anti-Her3/ErbB3 (Ab-1328) antibody A00539-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-01-29

Question

Is this A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody reactive to the isotypes of ERBB3?

A. Edwards

Verified customer

Asked: 2014-10-24

Answer

The immunogen of A00539-2 anti-Her3/ErbB3 (Ab-1328) antibody is Peptide sequence around aa. 1326~1330 (P-D-Y-W-H) derived from Human Her3/ErbB3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-10-24

Question

My colleagues were well pleased with the WB result of your anti-Her3/ErbB3 (Ab-1328) antibody. However we have been able to see positive staining in placenta small intestine isoform 1: cell membrane using this antibody. Is that expected? Could you tell me where is ERBB3 supposed to be expressed?

D. Parker

Verified customer

Asked: 2013-10-21

Answer

Based on literature, placenta small intestine does express ERBB3. Generally ERBB3 expresses in isoform 1: cell membrane. Regarding which tissues have ERBB3 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Hippocampus, Placenta, and Small intestine, Pubmed ID: 14702039
Liver, Pubmed ID: 24275569
Placenta, Pubmed ID: 7685162
Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2013-10-21

Size

Total: $299

SKU:A00539-2

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A00539-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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