Rabbit IgG polyclonal antibody for Endothelial PAS domain-containing protein 1(EPAS1) detection. Tested with WB, IHC-P in Rat. Cited in 1 publication(s).
Anti-HIF-2-alpha/EPAS1 Antibody Info At A Glance
|Product Name||Anti-HIF-2-alpha/EPAS1 Antibody
See all HIF-2 alpha/EPAS1 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for HIF 2 alpha/EPAS1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Rat. HIF 2 alpha/EPAS1 information: Molecular Weight: 96718 MW; Subcellular Localization: Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles.|
|Cite This Product||Anti-HIF-2-alpha/EPAS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1129-1)|
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|Predicted Reactivity||Bovine, Canine, Horse, Monkey, Rabbit
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at N-terminus of rat HIF-2-alpha(202-240aa YNNCPPHSSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLD).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Epas1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||29452|
|Species Of This Entry||Rat|
|Protein Name||Endothelial PAS domain-containing protein 1|
|Alternative Names||HIF-2 alpha/EPAS1|anti hif 2 alpha; HIF-2 alpha; Basic-helix-loop-helix-PAS protein MOP2; BHLHE73; Class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain protein 1; endothelial PAS domain-containing protein 1; EPAS1; EPAS-1; HIF 2A; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2 alpha; HIF-2 alpha; hif2a angiogenesis; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; Hypoxia-inducible factor 2-alpha; Member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2|
|Post Tranlational Modifications||Phosphorylation; Hydroxylation; Oxidation; Methylation|
|Gene Location||6q12, on Chromasome 6, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Epas1 (Source: Uniprot.org, NCBI)
|Protein Function||Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).|
|Research Areas||Angiogenesis; Apoptosis; Cancer; Cancer Stem Cells; Chromatin Research; HIF Target Genes; Hypoxia; Lipid and Metabolism; Transcription Factors and Regulators|
|Related Diseases||Hypoxia; Tumor Angiogenesis; Neoplasms; Malignant Neoplasms; Carcinoma; Anoxia; Pathologic Neovascularization; Cell Invasion; Renal Cell Carcinoma; Degenerative Polyarthritis; Pheochromocytoma; Altitude Sickness; Tissue Adhesions; Kidney Neoplasm; Hypertrophy; Tumor Progression; Obesity; Inflammation; Von Hippel-lindau Syndrome; Liver Carcinoma|
|Related Pathways||Angiogenesis; Response To Hypoxia; Pathogenesis; Transport; Cellular Response To Hypoxia; Cell Adhesion; Cell Differentiation; Cell Proliferation; Regeneration; Oxygen Homeostasis; Ossification; Endochondral Ossification; Regulation Of Angiogenesis; Glycolysis; Immune Response; Inflammatory Response; Reverse Transcription; Translation; Cell Cycle; Tissue Regeneration|
|Background||HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88% identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48% sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.|
|Scientific References||PMID: 11237857 by Kietzmann T., et al. Perivenous expression of the mRNA of the three hypoxia-inducible factor a-subunits HIF-1a, HIF2a and HIF3a in rat liver.|
Our Boster Quality Guarantee for Anti-HIF-2-alpha/EPAS1 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Rat Small Intestine Tissue at 1 ug/ml
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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