Data & Images
|Product Name||Anti-HIF-2-Alpha Antibody|
|Description||Rabbit IgG polyclonal antibody for Endothelial PAS domain-containing protein 1(EPAS1) detection. Tested with WB, IHC-P in Rat.|
|Cite This Product||Anti-HIF-2-Alpha Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1129-1)|
|Replacement Item||This antibody may replace the following items: sc-134359|sc-13596|sc-13596-X|sc-161712|sc-28706|sc-28706-X|sc-32146|sc-32146-X|sc-32147|sc-32147-X|sc-367607|sc-46691|sc-46691-X|sc-8712|sc-8712-X from Santa Cruz Biotechnology.|
|Predicted Species||Bovine, Canine, Horse, Monkey, Rabbit|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at N-terminus of rat HIF-2-alpha(202-240aa YNNCPPHSSLCGYKEPLLSCLIIMCEPIQHPSHMDIPLD).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Endothelial PAS domain-containing protein 1(EPAS-1)|
|Molecular Weight||96718 MW|
|Protein Function||Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity). .|
|Sequence Similarities||Contains 1 bHLH (basic helix-loop-helix) domain.|
|Subcellular Localization||Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .|
|Alternative Names||Endothelial PAS domain-containing protein 1;EPAS-1;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Epas1;Hif2a;|
|Research Areas|||cardiovascular|hypoxia|hif| epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper|hlh|cardiovascular/immune| cancer|invasion/microenvironment|chip'ing antibodies|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolic signaling pathways|nucleotide metabolism|molecular processes|mitochondrial transcription|metabolism processes|types of disease||
Background for Endothelial PAS domain-containing protein 1(EPAS-1)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HIF-2-Alpha Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, By Heat|
Western blot, 0.1-0.5μg/ml, Rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HIF-2-Alpha Antibody Images
Click the images to enlarge.
IHC(P): Rat Small Intestine Tissue at 1 ug/ml
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,