Anti-HIF-2 alpha Rabbit Monoclonal Antibody
|Reactivity||Human, Mouse, Rat|
|Applications||Flow Cytometry, IF, ICC, WB|
|Product Name||Anti-HIF-2 alpha Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-HIF-2 alpha Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, ICC, WB in Human, Mouse, Rat|
|Cite This Product||Anti-HIF-2 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01248)|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human HIF-2 alpha|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Flow Cytometry 1:50
Images And Assay Conditions
Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate (M01248).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPAS1 monoclonal antibody (Catalog # M01248) overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPAS1
Protein Target Info (Source: Uniprot.org)
|Protein Name||Endothelial PAS domain-containing protein 1|
|Tissue Specificity||Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.|
|Alternative Names||Endothelial PAS domain-containing protein 1;EPAS-1;Basic-helix-loop-helix-PAS protein MOP2;Class E basic helix-loop-helix protein 73;bHLHe73;HIF-1-alpha-like factor;HLF;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Member of PAS protein 2;PAS domain-containing protein 2;EPAS1;BHLHE73, HIF2A, MOP2, PASD2;|
|Subcellular Localization||Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .|
|Molecular Weight||96459 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.|
|Research Areas||Human, Mouse, Rat
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