Anti-HIF-2 alpha Rabbit Monoclonal Antibody


SKU M01248
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number ADD-5
Host Rabbit
Applications Flow Cytometry, IF, ICC, WB

Overview

Product Name Anti-HIF-2 alpha Rabbit Monoclonal Antibody
SKU/Catalog Number M01248
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-HIF-2 alpha Rabbit Monoclonal Antibody tested for Flow Cytometry, IF, ICC, WB in Human, Mouse, Rat
Cite This Product Anti-HIF-2 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01248)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human HIF-2 alpha
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration:
WB 1:500-1:2000
ICC/IF 1:50-1:200
Flow Cytometry 1:50

Images And Assay Conditions

Western blot analysis of HIF-2 alpha in (1)MCF-7 cell lysate;(2)HeLa cell lysate (M01248).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EPAS1 monoclonal antibody (Catalog # M01248) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EPAS1

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q99814
Gene Name EPAS1
Protein Name Endothelial PAS domain-containing protein 1
Tissue Specificity Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
Alternative Names Endothelial PAS domain-containing protein 1;EPAS-1;Basic-helix-loop-helix-PAS protein MOP2;Class E basic helix-loop-helix protein 73;bHLHe73;HIF-1-alpha-like factor;HLF;Hypoxia-inducible factor 2-alpha;HIF-2-alpha;HIF2-alpha;Member of PAS protein 2;PAS domain-containing protein 2;EPAS1;BHLHE73, HIF2A, MOP2, PASD2;
Subcellular Localization Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles. .
Molecular Weight 96459 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
Research Areas Human, Mouse, Rat

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Monoclonal antibody for HIF 2 alpha/EPAS1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IF, ICC, WB. Reactive species: Human, Mouse, Rat HIF 2 alpha/EPAS1 information: Molecular Weight: 96459 MW; Subcellular Localization: Nucleus . Nucleus speckle . Colocalizes with HIF3A in the nucleus and speckles; Tissue Specificity: Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
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In stock
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M01248
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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Publications

Downregulating hypoxia%u2010inducible factor%u20102%u03B1 improves the efficacy of doxorubicin in the treatment of hepatocellular carcinoma
Proper autophagy is indispensable for angiogenesis during chick embryo development
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