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SKU:PB9129
Clonality:Polyclonal
Application:WB
Price: $240.00
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Overview

Product Name Anti-HIF1 Beta Picoband™ Antibody
SKU/Catalog Number PB9129
Description Rabbit IgG polyclonal antibody for Aryl hydrocarbon receptor nuclear translocator(ARNT) detection. Tested with WB in Human.
Cite This Product Anti-HIF1 Beta Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9129)
Replacement Item This antibody may replace the following items: sc-161853|sc-161854|sc-17811|sc-17811-X|sc-17812|sc-17812-X|sc-271801|sc-271801-X|sc-366098|sc-393613|sc-393613-X|sc-393683|sc-393683-X|sc-393916|sc-55526|sc-5580|sc-5580-X|sc-5581|sc-5581-X|sc-56620|sc-65639|sc-70383|sc-8076|sc-8076-X|sc-8077|sc-8077-X|sc-8078|sc-8078-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human HIF1 beta recombinant protein (Position: V416-E789). Human HIF1 beta shares 86% and 83% amino acid (aa) sequences identity with mouse and rat HIF1 beta, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name HIF1A
Protein Name Aryl hydrocarbon receptor nuclear translocator
Molecular Weight 86636 MW
Protein Function Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
Sequence Similarities Contains 1 bHLH (basic helix-loop-helix) domain.
Subcellular Localization Nucleus.
Uniprot ID P27540
Alternative Names Aryl hydrocarbon receptor nuclear translocator;ARNT protein;Class E basic helix-loop-helix protein 2;bHLHe2;Dioxin receptor, nuclear translocator;Hypoxia-inducible factor 1-beta;HIF-1-beta;HIF1-beta;ARNT;BHLHE2;
Research Areas |neuroscience|neurology process|neural signal transduction| epigenetics and nuclear signaling|transcription|polymerase associated factors|pol ii transcription|transcription factors| cancer|cancer metabolism|response to hypoxia| metabolism|pathways and processes|metabolism processes|types of disease|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Aryl hydrocarbon receptor nuclear translocator

ARNT is also known as HIF1-beta or HIF1B. This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. It is mapped to 1q21.3. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. ARNT is a structural component of the XRE-binding form of the Ah receptor. It also functions in concert with RelB in a CD30-induced negative feedback mechanism.

Anti-HIF1 Beta Picoband™ Antibody Images

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Anti-HIF1 Beta Picoband™ Antibody
Anti-HIF1 beta Picoband antibody, PB9129-1.jpg
All lanes: Anti HIF1 beta (PB9129) at 0.5ug/ml
WB: Recombinant Human HIF1 beta Protein 0.5ng
Predicted bind size: 49KD
Observed bind size: 49KD
Anti-HIF1 Beta Picoband™ Antibody
Anti-HIF1 beta Picoband antibody, PB9129-2.jpg
All lanes: Anti HIF1 beta (PB9129) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: Jurkat Whole Cell Lysate at 40ug
Lane 4: U87 Whole Cell Lysate at 40ug
Lane5: Colo320 Whole Cell Lysate at 40ug
Predicted bind size: 87KD
Observed bind size: 87KD
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to arnt antibody, hif 1 beta antibody, hif1-beta antibody, hif-1-beta antibody