Anti-His Tag Antibody (monoclonal, 9C11)

Tmem177 antibody

Boster Bio Anti-His Tag Antibody (monoclonal, 9C11) catalog # M30975. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species. Cited in 10 publication(s).

Product Info Summary

SKU: M30975
Size: 100μl/vial
Reactive Species: All species
Host: Mouse
Application: Dot blot, ELISA, WB

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Product Name

Anti-His Tag Antibody (monoclonal, 9C11)

View all Tmem177 Antibodies

SKU/Catalog Number

M30975

Size

100μl/vial

Form

Liquid

Description

Boster Bio Anti-His Tag Antibody (monoclonal, 9C11) catalog # M30975. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-His Tag Antibody (monoclonal, 9C11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30975)

Host

Mouse

Contents

0.5mg/ml in PBS, pH7.4, containing 50% glycerol.

Clonality

Monoclonal

Clone Number

9C11

Isotype

IgG1

Immunogen

This antibody is produced by immunizing animals with a synthetic His tag peptide corresponding to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M30975 is reactive to Tmem177 in All species

Applications

M30975 is guaranteed for Dot blot, ELISA, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

34.067kDa

Background of Tmem177

A Polyhistidine-Tag is an amino acids motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-Tag, 6xHis-Tag, His6 Tag and by the trademarked name His-Tag. Polyhistidine-Tags are often used for affinity purification of genetically modified proteins.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

1:500-1:5000

Validation Images & Assay Conditions

Gene/Protein Information For Tmem177 (Source: Uniprot.org, NCBI)

Gene Name

Tmem177

Full Name

Transmembrane protein 177

Weight

34.067kDa

Superfamily

TMEM177 family

Alternative Names

MGC10993; transmembrane protein 177

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Tmem177, check out the Tmem177 Infographic

Tmem177 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Tmem177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M30975 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation

Development of an indirect ELISA based on a truncated S protein of the porcine epidemic diarrhea virus

The 656C and 725C are two important sites in gene STGC3 for its negative regulation on cell growth

Fibronectin-/fibrinogen-binding protein (FBPS) is not a critical virulence factor for the Streptococcus suis serotype 2 strain ZY05719

Equine adipose-derived stem cell (ASC) expresses BAFF and its receptors, which may be associated with the differentiation process of ASC towards adipocyte

Molecular characterization and evolutionary analysis of horse BAFF-R, a tumor necrosis factor receptor related to B-cell survival

Characterization and bioactivity of grass carp (Ctenopharyngodon idella) interleukin-21: Inducible production and involvement in inflammatory regulation

Identification and functional characterization of tumor necrosis factor receptor 1 (TNFR1) of grass carp (Ctenopharyngodon idella)

Efficient yeast cell-surface display of an endoglucanase of Aspergillus flavus and functional characterization of the whole-cell enzyme

High-level expression and purification of Tat-haFGF 19-154

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2 Customer Q&As for Anti-His Tag Antibody (monoclonal, 9C11)

Question

Does M30975 have the strongest affinity?

Verified customer

Asked: 2019-03-21

Answer

Yes, the Anti-His Tag Antibody (Monoclonal, 9C11) (M30975) has the strongest affinity.

Boster Scientific Support

Answered: 2019-03-26

Question

Can M30975 be used to detect for the shorter his tag (4-His)?

Verified Customer

Verified customer

Asked: 2019-03-11

Answer

M30975 has not been tested to detect the shorter his tag (4-his).

Boster Scientific Support

Answered: 2019-03-11

Order DetailsPrice
M30975

100uL

$299

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