Product Info Summary
SKU: | M30975 |
---|---|
Size: | 100μl/vial |
Reactive Species: | All species |
Host: | Mouse |
Application: | Dot blot, ELISA, WB |
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Product info
Product Name
Anti-His Tag Antibody (monoclonal, 9C11)
SKU/Catalog Number
M30975
Size
100μl/vial
Form
Liquid
Description
Boster Bio Anti-His Tag Antibody (monoclonal, 9C11) catalog # M30975. Tested in Dot blot, ELISA, WB applications. This antibody reacts with All species.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-His Tag Antibody (monoclonal, 9C11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30975)
Host
Mouse
Contents
0.5mg/ml in PBS, pH7.4, containing 50% glycerol.
Clonality
Monoclonal
Clone Number
9C11
Isotype
IgG1
Immunogen
This antibody is produced by immunizing animals with a synthetic His tag peptide corresponding to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M30975 is reactive to Tmem177 in All species
Applications
M30975 is guaranteed for Dot blot, ELISA, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
34.067kDa
Background of Tmem177
A Polyhistidine-Tag is an amino acids motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-Tag, 6xHis-Tag, His6 Tag and by the trademarked name His-Tag. Polyhistidine-Tags are often used for affinity purification of genetically modified proteins.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
1:500-1:5000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of different concentration of Anti-His tag (9C11) Monoclonal Antibody
Antigen: Extracts from E.coli expressing His-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ℃ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins
Protein Target Info & Infographic
Gene/Protein Information For Tmem177 (Source: Uniprot.org, NCBI)
Gene Name
Tmem177
Full Name
Transmembrane protein 177
Weight
34.067kDa
Superfamily
TMEM177 family
Alternative Names
MGC10993; transmembrane protein 177
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Tmem177, check out the Tmem177 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Tmem177: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-His Tag Antibody (monoclonal, 9C11) (M30975)
Hello CJ!
M30975 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation
Development of an indirect ELISA based on a truncated S protein of the porcine epidemic diarrhea virus
The 656C and 725C are two important sites in gene STGC3 for its negative regulation on cell growth
Fibronectin-/fibrinogen-binding protein (FBPS) is not a critical virulence factor for the Streptococcus suis serotype 2 strain ZY05719
Equine adipose-derived stem cell (ASC) expresses BAFF and its receptors, which may be associated with the differentiation process of ASC towards adipocyte
Molecular characterization and evolutionary analysis of horse BAFF-R, a tumor necrosis factor receptor related to B-cell survival
Characterization and bioactivity of grass carp (Ctenopharyngodon idella) interleukin-21: Inducible production and involvement in inflammatory regulation
Identification and functional characterization of tumor necrosis factor receptor 1 (TNFR1) of grass carp (Ctenopharyngodon idella)
Efficient yeast cell-surface display of an endoglucanase of Aspergillus flavus and functional characterization of the whole-cell enzyme
High-level expression and purification of Tat-haFGF 19-154
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Customer Reviews
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Customer Q&As
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2 Customer Q&As for Anti-His Tag Antibody (monoclonal, 9C11)
Question
Does M30975 have the strongest affinity?
Verified customer
Asked: 2019-03-21
Answer
Yes, the Anti-His Tag Antibody (Monoclonal, 9C11) (M30975) has the strongest affinity.
Boster Scientific Support
Answered: 2019-03-26
Question
Can M30975 be used to detect for the shorter his tag (4-His)?
Verified Customer
Verified customer
Asked: 2019-03-11
Answer
M30975 has not been tested to detect the shorter his tag (4-his).
Boster Scientific Support
Answered: 2019-03-11