Anti-HLA-B Antibody Picoband™

Product Name

Anti-HLA-B Antibody Picoband™

See all HLA-B products

SKU/Catalog Number

A00186-1

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA-B Antibody Picoband™ catalog # A00186-1. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA-B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00186-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-B (52-89aa EQEGPEYWDRNTQIFKTNTQTYRENLRIALRYYNQSEA).

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00186-1 is reactive to HLA-B in Human

Applications

A00186-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of HLA-B using anti-HLA-B antibody (A00186-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HELA whole Cell lysates,
Lane 2: HEPG2 whole Cell lysates,
Lane 3: SGC7901 whole Cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-B antigen affinity purified polyclonal antibody (Catalog # A00186-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-B at approximately 45KD. The expected band size for HLA-B is at 45KD.

Gene/Protein Information For HLA-B (Source: Uniprot.Org, NCBI)

Alternative Names

ankylosing spondylitis; AS; b'DT; Bw-22; Bw-4; Bw-41; Bw-44; Bw-45; Bw-46; Bw-47; Bw-48; Bw-50; Bw-52; Bw-53; Bw-54; Bw-55; Bw-56; Bw-57; Bw-58; Bw-60; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, B-12 alpha chain; HLA class I histocompatibility antigen, B-21 alpha chain; HLA class I histocompatibility antigen, B-5 alpha chain; HLA class I histocompatibility antigen, B-7 alpha chain; HLAB; HLA-B27; HLA-B73; leukocyte antigen class I-B; lymphocyte antigen; major histocompatibility complex, class I, B; MGC111087; MHC class I antigen B*13; MHC cla HLA-B AS, B-4901, HLAB major histocompatibility complex, class I, B major histocompatibility complex, class I, B|HLA class I antigen HLA-B|HLA class I histocompatibility antigen, B alpha chain|MHC HLA-B cell surface glycoprotein|MHC HLA-B transmembrane glycoprotein|MHC class 1 antigen|MHC class I antigen HLA-B alpha chain|MHC class I antigen HLA-B heavy chain|MHC class I antigen SHCHA|MHC class I molecule|leukocyte antigen class I-B

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In this infographic you will see the following information for HLA-B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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