Data & Images
|Product Name||Anti-HLA DMB Antibody|
|Description||Rabbit IgG polyclonal antibody for HLA class II histocompatibility antigen, DM beta chain(HLA-DMB) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-HLA DMB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1788)|
|Replacement Item||This antibody may replace the following items: sc-14539|sc-14540|sc-367765|sc-393548|sc-69738 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human HLA DMB(81-100aa NQKDTLMQRLRNGLQNCATH).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||HLA class II histocompatibility antigen, DM beta chain|
|Molecular Weight||28943 MW|
|Protein Function||Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. .|
|Sequence Similarities||Belongs to the MHC class II family.|
|Subcellular Localization||Late endosome membrane ; Single-pass type I membrane protein . Lysosome membrane ; Single-pass type I membrane protein . Localizes to late endocytic compartment. Associates with lysosome membranes.|
|Alternative Names||HLA class II histocompatibility antigen, DM beta chain;MHC class II antigen DMB;Really interesting new gene 7 protein;HLA-DMB;DMB, RING7;|
|Research Areas|||immunology|adaptive immunity|mhc|class ii||
Background for HLA class II histocompatibility antigen, DM beta chain
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HLA DMB Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HLA DMB Antibody Images
Click the images to enlarge.
All lanes: Anti HLA DMB (PA1788) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: JURKAT Whole Cell Lysate at 40ug
Lane 3: RAJI Whole Cell Lysate at 40ug
Lane 4: HUT Whole Cell Lysate at 40ug
Predicted bind size: 29KD
Observed bind size: 29KD
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,