|Application:||IHC-P, ICC, WB|
Data & Images
|Product Name||Anti-HSD17B1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Estradiol 17-beta-dehydrogenase 1(HSD17B1) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.|
|Cite This Product||Anti-HSD17B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1613-1)|
|Replacement Item||This antibody may replace the following items: sc-100453|sc-26963|sc-26964|sc-26965|sc-32871|sc-32872|sc-365888|sc-373902|sc-376719 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human HSD17B1(29-43aa QSFKVYATLRDLKTQ), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Estradiol 17-beta-dehydrogenase 1|
|Molecular Weight||34950 MW|
|Protein Function||Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.|
|Sequence Similarities||Belongs to the short-chain dehydrogenases/reductases (SDR) family.|
|Alternative Names||Estradiol 17-beta-dehydrogenase 1;22.214.171.124;17-beta-hydroxysteroid dehydrogenase type 1;17-beta-HSD 1;20 alpha-hydroxysteroid dehydrogenase;20-alpha-HSD;E2DH;Placental 17-beta-hydroxysteroid dehydrogenase;Short chain dehydrogenase/reductase family 28C member 1;HSD17B1;E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1;|
|Research Areas|||signal transduction|growth factors/hormones| signal transduction|metabolism|energy metabolism| neuroscience|endocrine system|gonadotrophic axis| cancer|growth factors| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|types of disease||
Background for Estradiol 17-beta-dehydrogenase 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HSD17B1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-HSD17B1 Antibody Images
Click the images to enlarge.
ICC: HELA Cell
Lane 1: Rat Kidney Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: 293T Cell Lysate
Lane 4: HELA Cell Lysate
IHC(P): Rat Liver Tissue
IHC(P): Rat Ovary Tissue
IHC(P): Human Placenta Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,