Anti-Hsp20/HSPB6 Antibody Picoband™

HSP20/HSPB6 antibody

Boster Bio Anti-Hsp20/HSPB6 Antibody Picoband™ catalog # A07981-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A07981-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

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Product Name

Anti-Hsp20/HSPB6 Antibody Picoband™

View all HSP20/HSPB6 Antibodies

SKU/Catalog Number

A07981-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp20/HSPB6 Antibody Picoband™ catalog # A07981-1. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp20/HSPB6 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07981-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Hsp20 recombinant protein (Position: H73-L123). Human Hsp20 shares 88.2% and 92.2% amino acid (aa) sequence identity with mouse and rat Hsp20, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07981-1 is reactive to HSPB6 in Human, Mouse, Rat

Applications

A07981-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

17.136kDa

Background of HSP20/HSPB6

Heat shock protein beta-6 is a protein that in humans is encoded by the HSPB6 gene. HSPB6, also known as hsp20, is a 17-kDa member of the small heat shock family of proteins. It is expressed in multiple tissues; however, HSPB6 is most highly and constitutively expressed in different types of muscle including vascular, airway, colonic, bladder, uterine smooth muscle, cardiac muscle and skeletal muscle. HSPB6 has specific functions for vasodilation, platelet function, and insulin resistance and in smooth and cardiac muscle.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSPB6 (Source: Uniprot.org, NCBI)

Gene Name

HSPB6

Full Name

Heat shock protein beta-6

Weight

17.136kDa

Superfamily

small heat shock protein (HSP20) family

Alternative Names

FLJ32389; Heat shock 20 kDa-like protein p20; heat shock protein beta-6; heat shock protein, alpha-crystallin-related, B6; HSP20; HSPB6 HSPB6 HEL55, Hsp20, PPP1R91 heat shock protein family B (small) member 6 heat shock protein beta-6|epididymis luminal protein 55|epididymis secretory sperm binding protein|heat shock 20 kDa-like protein p20|heat shock protein family B (small) member B6|heat shock protein, alpha-crystallin-related, B6|protein phosphatase 1, regulatory subunit 91

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPB6, check out the HSPB6 Infographic

HSPB6 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPB6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Hsp20/HSPB6 Antibody Picoband™

Question

Would A07981-1 anti-Hsp20/HSPB6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

It shows on the product datasheet, A07981-1 anti-Hsp20/HSPB6 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-02-04

Question

We are interested in to test anti-Hsp20/HSPB6 antibody A07981-1 on mouse muscle for research purposes, then I may be interested in using anti-Hsp20/HSPB6 antibody A07981-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

The products we sell, including anti-Hsp20/HSPB6 antibody A07981-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-07

Question

We are currently using anti-Hsp20/HSPB6 antibody A07981-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

The anti-Hsp20/HSPB6 antibody (A07981-1) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-05-09

Question

Is a blocking peptide available for product anti-Hsp20/HSPB6 antibody (A07981-1)?

Verified Customer

Verified customer

Asked: 2018-01-25

Answer

We do provide the blocking peptide for product anti-Hsp20/HSPB6 antibody (A07981-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-01-25

Question

Can you help my question with product A07981-1, anti-Hsp20/HSPB6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-09-28

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07981-1 anti-Hsp20/HSPB6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-09-28

Question

Does anti-Hsp20/HSPB6 antibody A07981-1 work on dog WB with heart left ventricle?

Verified Customer

Verified customer

Asked: 2017-08-04

Answer

Our lab technicians have not tested anti-Hsp20/HSPB6 antibody A07981-1 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Hsp20/HSPB6 antibody A07981-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog heart left ventricle in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-04

Question

Does anti-Hsp20/HSPB6 antibody A07981-1 work for WB with muscle?

A. Evans

Verified customer

Asked: 2013-02-21

Answer

According to the expression profile of muscle, HSPB6 is highly expressed in muscle. So, it is likely that anti-Hsp20/HSPB6 antibody A07981-1 will work for WB with muscle.

Boster Scientific Support

Answered: 2013-02-21

Order DetailsPrice
A07981-1

100μg

$370
A07981-1-10ug

10μg sample (liquid)

$99
A07981-1-Biotin

100 μg Biotin conjugated

$570
A07981-1-Cy3

100 μg Cy3 conjugated

$570
A07981-1-Dylight488

100 μg Dylight488 conjugated

$570
A07981-1-Dylight550

100 μg Dylight550 conjugated

$570
A07981-1-Dylight594

100 μg Dylight594 conjugated

$570
A07981-1-FITC

100 μg FITC conjugated

$570
A07981-1-HRP

100 μg HRP conjugated

$570
A07981-1-APC

100 μg APC conjugated

$670
A07981-1-PE

100 μg PE conjugated

$670
A07981-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A07981-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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