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SKU:MA1048
Clonality:Monoclonal
Clone Number:IAP-9
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-HSP27 Antibody
SKU/Catalog Number MA1048
Description Mouse IgG monoclonal antibody for HSP27, heat shock 27kDa protein 1 (HSPB1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;rat;rabbit. No cross reactivity with other proteins.
Cite This Product Anti-HSP27 Antibody IAP-9 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1048)
Replacement Item This antibody may replace the following items: sc-53718 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rabbit, Rat
Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Partially purified inhibitor of actin polymerization(IAP) protein from turkey gizzard smooth muscle.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number IAP-9
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Hspb1
Protein Name Heat shock protein beta-1
Molecular Weight 22893 MW
Protein Function Involved in stress resistance and actin organization.
Tissue Specificity Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris and extensor digitorum muscles. .
Sequence Similarities Belongs to the small heat shock protein (HSP20) family.
Subcellular Localization Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, spindle . Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles (By similarity). .
Uniprot ID P42930
Alternative Names Heat shock protein beta-1;HspB1;Heat shock 27 kDa protein;HSP 27;Hspb1;Hsp27;
Research Areas |signal transduction|protein trafficking|chaperones|heat shock proteins| cancer|tumor biomarkers| cardiovascular|atherosclerosis|ischemia / reperfusion|heart|contractility|contractile proteins|actins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Heat shock protein beta-1

The heat-shock proteins(HSPs) belong to a larger group of polypeptides, the stress proteins, that are induced in various combinations in response to environmental challenges and developmental transitions. Heat-shock 27-kD protein1also knows as HSPB. Synthesis of the small(27-kD) HSP has been shown to be correlated with the acquisition of thermotolerance. HSP27 gene is mapped to 7q11.23. Mutant small heat-shock protein 27 causes axonal Charcot-Marie-Tooth disease and distal hereditary motor neuropathy. Heat shock protein 27 prevents cellular polyglutamine toxicity and suppresses the increase of reactive oxygen species caused by huntingtin.
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Publications

Wu Yw, Hao T, Kou Xx, Gan Yh, Ma Xc. Arch Oral Biol. 2015 Sep;60(9):1310-8. Doi: 10.1016/J.Archoralbio.2015.05.011. Epub 2015 Jun 11. Synovial Trpv1 Is Upregulated By 17-??-Estradiol And Involved In Allodynia Of Inflamed Temporomandibular Joints In...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.