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SKU:MA1049
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:LK1
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-HSP60 Antibody (Monoclonal, LK1)
SKU/Catalog Number MA1049
Description Mouse IgG monoclonal antibody for HSP60, heat shock 60kDa protein 1 (chaperonin) (HSPD1) detection. Tested with WB, IHC-P in Human;rat;chicken. No cross reactivity with other proteins.
Cite This Product Anti-HSP60 Antibody (Monoclonal, LK1) LK1 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1049)
Replacement Item This antibody may replace the following items: sc-1052|sc-13115|sc-136291|sc-13966|sc-1722|sc-1722-R|sc-271215|sc-376240|sc-376261|sc-59567|sc-65568 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Recombinant human heat shock protein 60(HSP60)
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number LK1
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Hspd1
Protein Name 60 kDa heat shock protein, mitochondrial
Molecular Weight 60955 MW
Protein Function Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Sequence Similarities Belongs to the chaperonin (HSP60) family.
Subcellular Localization Mitochondrion matrix .
Uniprot ID P63039
Alternative Names 60 kDa heat shock protein, mitochondrial;60 kDa chaperonin;Chaperonin 60;CPN60;HSP-65;Heat shock protein 60;HSP-60;Hsp60;Mitochondrial matrix protein P1;Hspd1;Hsp60;
Research Areas |tags & cell markers|subcellular markers|organelles|mitochondria| signal transduction|protein trafficking|chaperones|heat shock proteins| kits/ lysates/ other|elisa kits|signal transduction proteins elisa kits| isotype/loading controls|hsp60|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for 60 kDa heat shock protein, mitochondrial

Heat shock 60KD protein(HSP60) is a member of the chaperonin class of protein factors and the nuclear encoded mitochondrial HSP60 is required for the assembly into oligomeric complexes of proteins imported into the mitochondrial matrix. HSP60 is linked head to head comprising approximately 17 kb and consist of 12 exons. HSP60 is a self-molecule, it can downregulate adaptive immune responses by upregulating Tregs innately through TLR2 signaling.
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Publications

Cui Yd, Du Yz, Lu Mx, Qiang Ck. J Insect Sci. 2010;10:100. Doi: 10.1673/031.010.10001. Cloning Of The Heat Shock Protein 60 Gene From The Stem Borer, Chilo Suppressalis, And Analysis Of Expression Characteristics Under Heat Stress.
Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...
Li L, Nan P, Zhai S, Wang L, Si S, Chang Z. Mol Cell Biochem. 2014 Feb;387(1-2):143-50. Doi: 10.1007/S11010-013-1879-0. Epub 2013 Nov 21. Molecular Cloning, Characterization, And Expression Of Hsp60 In Caudal Fin Regeneration Of Misgurnus Anguilli...
Li L, Zhai S, Wang L, Si S, Wu H, Chang Z. Fish Shellfish Immunol. 2014 Feb;36(2):401-8. Doi: 10.1016/J.Fsi.2013.12.016. Epub 2013 Dec 29. Hsp60 In Caudal Fin Regeneration From Paramisgurnus Dabryanus: Molecular Cloning And Expression Characteriza...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to hsp60 antibody, groel antibody