Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody

HSP60 antibody

Boster Bio Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody (Catalog # M01280). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey, Chicken, Xenopus laevis, Drosophila. Cited in 3 publication(s).

Product Info Summary

SKU: M01280
Size: 100ug/vial
Reactive Species: Chicken, Cow, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

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Product Name

Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody

View all HSP60 Antibodies

SKU/Catalog Number

M01280

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody (Catalog # M01280). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Hamster, Sheep, Rabbit, Cow, Dog, Pig, Monkey, Chicken, Xenopus laevis, Drosophila.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01280)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM253

Isotype

IgG1, kappa

Immunogen

Recombinant human HSP60 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01280 is reactive to HSPD1 in Chicken, Cow, Dog, Drosophila, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis

Applications

M01280 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

61.055kDa

Background of HSP60

Recognizes a 60kDa protein, identified as the heat shock protein 60 (hsp60). Its epitope is localized between aa 383-447 of human hsp60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). hsp60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial hsp60, but also against its mammalian homolog.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For HSPD1 (Source: Uniprot.org, NCBI)

Gene Name

HSPD1

Full Name

60 kDa heat shock protein, mitochondrial

Weight

61.055kDa

Superfamily

chaperonin (HSP60) family

Alternative Names

60 kDa chaperonin; Chaperonin 60; cpn60; GROEL; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); Heat shock protein 60; heat shock protein 65; HLD4; HSP60; HSP-60; HSP60SPG13; HSP65; HSPD1; HuCHA60; Mitochondrial matrix protein P1,60 kDa heat shock protein, mitochondrial; P60 lymphocyte protein; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant); SPG13 HSPD1 CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13 heat shock protein family D (Hsp60) member 1 60 kDa heat shock protein, mitochondrial|60 kDa chaperonin|P60 lymphocyte protein|chaperonin 60|epididymis secretory sperm binding protein|heat shock 60kDa protein 1 (chaperonin)|heat shock protein 65|mitochondrial matrix protein P1|short heat shock protein 60 Hsp60s1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPD1, check out the HSPD1 Infographic

HSPD1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M01280 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression and location of HSP60 and HSP10 in the heart tissue of heat-stressed rats

Hepatic mitochondrial and ER stress induced by defective PPAR? signaling in the pathogenesis of hepatic steatosis

Hager L, Li L, Pun H, Liu L, Hossain Ma, Maguire Gf, Naples M, Baker C, Magomedova L, Tam J, Adeli K, Cummins Cl, Connelly Pw, Ng Ds. J Biol Chem. 2012 Jun 8;287(24):20755-68. Doi: 10.1074/Jbc.M112.340919. Epub 2012 Apr 12. Lecithin:Cholesterol Ac...

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6 Customer Q&As for Anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal Antibody

Question

Is this M01280 anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody reactive to the isotypes of HSPD1?

Verified Customer

Verified customer

Asked: 2020-02-17

Answer

The immunogen of M01280 anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody is Recombinant human HSP60 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-17

Question

Does anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody M01280 work for IHC with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-10-09

Answer

According to the expression profile of cervix carcinoma, HSPD1 is highly expressed in cervix carcinoma. So, it is likely that anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody M01280 will work for IHC with cervix carcinoma.

Boster Scientific Support

Answered: 2019-10-09

Question

Here is the WB image, lot number and protocol we used for cervix carcinoma using anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody M01280. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-09-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-02

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody M01280. Let me know if you need anything else.

O. Johnson

Verified customer

Asked: 2018-10-31

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-10-31

Question

Is a blocking peptide available for product anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody (M01280)?

C. Krishna

Verified customer

Asked: 2014-09-23

Answer

We do provide the blocking peptide for product anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody (M01280). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2014-09-23

Question

Do you have a BSA free version of anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody M01280 available?

S. Rodriguez

Verified customer

Asked: 2014-01-29

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-HSP60 (Heat Shock Protein 60) (Mitochondrial Marker) Monoclonal antibody M01280 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-01-29

Order DetailsPrice
M01280-100ug

100 μg

$468

Free Secondary Antibody

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M01280
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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