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- Table of Contents
HSPD1 antibody (PB9337) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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Anti-Hsp60 Picoband antibody, PB9337, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Designing a Western blot for HSPD1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the HSPD1 Western Blot GuideBundle Save
Anti-Hsp60/HSPD1 Antibody Picoband®
HSPD1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
HSPD1 antibody was positively detected by WB in:
human Hela whole cell, human A549 whole cell, human PANC-1 whole cell, human MCF-7 whole cell, rat liver tissue, mouse liver tissue, mouse thymus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
HSPD1 antibody was positively detected by IHC in:
Mouse Intestine Tissue, Rat Intestine Tissue, Human Intestinal Cancer Tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HSPD1 antibody was positively detected by ICC/IF in:
U20S cell
HSPD1 antibody was positively detected by ICC in:
SMMC-7721 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Hsp60/HSPD1 Antibody Picoband®
View all HSPD1 Antibodies
View all HSPD1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Hsp60 recombinant protein (Position: A260-Q496). Human Hsp60 shares 97% amino acid (aa) sequence identity with both mouse and rat Hsp60.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
61 kDa
Calculated Molecular Weight
61.1 kDa
Background of HSPD1
HSP60 is a member of the chaperonin class of protein factors, which include the Escherichia coli groEL protein and the Rubisco subunit-binding protein of chloroplasts. It acts as a costimulator of human regulatory CD4-positive/CD25 -positive T cells, which inhibit lymphoproliferation and IFNG and TNF secretion by CD4-positive and CD8-positive T cells. HSP60 enhances Treg activity via TLR2, leading to activation of an intracellular signaling cascade that included p38, as well as inhibition of ERK phosphorylation. Suppression of target T cells is mediated by both cell-to-cell contact and by secretion of TGFB and IL10, and it leads to downregulation of ERK, NFKB, and TBET expression. The self-molecule HSP60 can downregulate adaptive immune responses by upregulating Tregs through TLR2 signaling.
Cite This Product
Anti-Hsp60/HSPD1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9337)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Rabbit
99%
High Homology
Dog
99%
High Homology
Monkey
98%
High Homology
Sheep
98%
High Homology
Cow
97%
High Homology
Mouse
97%
High Homology
Rat
97%
High Homology
Zebrafish
84%
Moderate Homology
Drosophila
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for HSPD1 antibody
Immunohistochemistry (IHC) protocol for HSPD1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HSPD1 antibody
Immunocytochemistry (ICC) protocol for HSPD1 antibody
Publications
Application:
Species:
An experimental rat model of electric shock injury with isolated electric shock and water conduction: the histopathological changes on the skin and internal organs and the effect on biochemical parameters
Published: 2022-05-07
Journal: INTERNATIONAL JOURNAL OF LEGAL MEDICINE
Application: IHC
Impact Factor: 2.79
Species: Rat
PMID: 35524806
Denatured corona proteins mediate the intracellular bioactivities of nanoparticles via the unfolded protein response
Published: 2020-10-16
Journal: BIOMATERIALS
Application: WB
Impact Factor: 10.32
Species: Mouse
PMID: 33190736
Cloning of the heat shock protein 60 gene from the stem borer, Chilo suppressalis, and analysis of expression characteristics under heat stress
Published: 2010-01-01
Journal: JOURNAL OF INSECT SCIENCE
Application: IF
Impact Factor: 1.07
Species: C. suppressalis
PMID: 20673188
Review
Have you used Anti-Hsp60/HSPD1 Antibody Picoband®?
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1 Review For Anti-Hsp60/HSPD1 Antibody Picoband®
Western blot validation using three pairs of human cervical cancer and adjacent tissues showed that HSP60 antibody (PB9337) produced a clear target band, with higher HSP60 expression observed in cancer tissues compared with adjacent tissues.
| SKU | PB9337 |
|---|---|
| Application | Western Blot |
| Sample | human cervical cancer and adjacent tissue |
| Sample Processing Description | Total proteins were extracted from three human cervical cancer tissues and three adjacent normal tissues. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | Hsp60/HSPD1 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1 hour in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | This experiment aimed to evaluate the expression difference of HSP60 between cervical cancer and adjacent tissues. The results from three paired samples showed that HSP60 expression was lower in adjacent tissues than in cancer tissues. |
Xiaoyuan Qu, Shandong First Medical University
Verified customer
Submitted 2026-07-07
Product Promise
We promise Anti-Hsp60/HSPD1 Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9337
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 17, 2026