Anti-HSP70 HSPA4 Monoclonal Antibody

HSPA4 antibody

Boster Bio Anti-HSP70 HSPA4 Monoclonal Antibody catalog # A03618. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A03618
Size: 100μl
Reactive Species: Human
Host: Mouse
Application: IF, IHC, WB

Product Name

Anti-HSP70 HSPA4 Monoclonal Antibody

View all HSPA4 Antibodies

SKU/Catalog Number

A03618

Size

100μl

Form

Liquid

Description

Boster Bio Anti-HSP70 HSPA4 Monoclonal Antibody catalog # A03618. Tested in IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HSP70 HSPA4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03618)

Host

Mouse

Contents

Ascitic fluid containing 0.03% sodium azide.

Clonality

Monoclonal

Clone Number

5A6

Isotype

Antiserum

Immunogen

Purified recombinant fragment of human HSP70 expressed in E. Coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03618 is reactive to HSPA4 in Human

Applications

A03618 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

94.331kDa

Background of HSPA4

CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCF complex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as an E3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentially regulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a central region of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and is dissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC, 1:200-1:1000
IF, 1:200-1:1000

Validation Images & Assay Conditions

Gene/Protein Information For HSPA4 (Source: Uniprot.org, NCBI)

Gene Name

HSPA4

Full Name

Heat shock 70 kDa protein 4

Weight

94.331kDa

Superfamily

heat shock protein 70 family

Alternative Names

APG2; APG-2; heat shock 70 kDa protein 4; heat shock 70kD protein 4; heat shock 70kDa protein 4; Heat shock 70-related protein APG-2; heat shock protein, 110 kDa; HS24/P52; hsp70; hsp70RY; MGC131852; RY HSPA4 APG-2, HEL-S-5a, HS24/P52, HSPH2, RY, hsp70, hsp70RY heat shock protein family A (Hsp70) member 4 heat shock 70 kDa protein 4|epididymis secretory sperm binding protein Li 5a|heat shock 70-related protein APG-2|heat shock 70kD protein 4|heat shock protein, 110 kDa|hsp70 RY

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPA4, check out the HSPA4 Infographic

HSPA4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-HSP70 HSPA4 Monoclonal Antibody

Question

I was wanting to use your anti-HSP70 Monoclonal antibody for IF for human testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human testis identification?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

You can see on the product datasheet, A03618 anti-HSP70 Monoclonal antibody has been validated for IF, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-17

Question

Please see the WB image, lot number and protocol we used for testis using anti-HSP70 Monoclonal antibody A03618. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-23

Question

I was wanting to use to test anti-HSP70 Monoclonal antibody A03618 on human testis for research purposes, then I may be interested in using anti-HSP70 Monoclonal antibody A03618 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-16

Answer

The products we sell, including anti-HSP70 Monoclonal antibody A03618, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-16

Question

Does anti-HSP70 Monoclonal antibody A03618 work for IF with testis?

Verified Customer

Verified customer

Asked: 2019-02-21

Answer

According to the expression profile of testis, HSPA4 is highly expressed in testis. So, it is likely that anti-HSP70 Monoclonal antibody A03618 will work for IF with testis.

Boster Scientific Support

Answered: 2019-02-21

Question

Is a blocking peptide available for product anti-HSP70 Monoclonal antibody (A03618)?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

We do provide the blocking peptide for product anti-HSP70 Monoclonal antibody (A03618). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-02-11

Question

I have a question about product A03618, anti-HSP70 Monoclonal antibody . I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-07

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03618 anti-HSP70 Monoclonal antibody , we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-07

Question

Would anti-HSP70 Monoclonal antibody A03618 work on primate IHC with cervix carcinoma erythroleukemia?

Verified Customer

Verified customer

Asked: 2018-08-06

Answer

Our lab technicians have not validated anti-HSP70 Monoclonal antibody A03618 on primate. You can run a BLAST between primate and the immunogen sequence of anti-HSP70 Monoclonal antibody A03618 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma erythroleukemia in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-08-06

Order DetailsPrice
A03618

100uL

$399

Free Secondary Antibody

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A03618
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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