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SKU:MA1050
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:BRM-22
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-HSP70 Antibody (Monoclonal, BRM-22)
SKU/Catalog Number MA1050
Description Mouse IgG monoclonal antibody for HSP70, heat shock 70kDa protein 1A; heat shock 70kDa protein 1B (HSPA1A; HSPA1B) detection. Tested with WB, IHC-P, IHC-F in Human;bovine;rabbit;rat. No cross reactivity with other proteins.
Cite This Product Anti-HSP70 Antibody (Monoclonal, BRM-22) BRM-22 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)
Replacement Item This antibody may replace the following items: sc-33575|sc-59572 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Bovine, Human, Rabbit, Rat
Predicted Species Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen HSP70 isolated from bovine brain.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number BRM-22
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Hspa1b
Protein Name Heat shock 70 kDa protein 1A/1B
Molecular Weight 70176 MW
Protein Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. .
Tissue Specificity Testis-specific.
Sequence Similarities Belongs to the heat shock protein 70 family.
Subcellular Localization Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .
Uniprot ID P17879
Alternative Names Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1;
Research Areas signal transduction|protein trafficking|chaperones|heat shock proteins|cancer|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Heat shock 70 kDa protein 1A/1B

Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex(MHC) class III region 92 kilobases(kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.
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Publications

Bharati, J., Dangi, S., Chouhan, V. et al. Int J Biometeorol (2016). doi:10.1007/s00484-016-1281-1 Expression dynamics of HSP70 during chronic heat stress in Tharparkar cattle
Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X. Chemosphere. 2013 May;91(8):1113-22. Doi: 10.1016/J.Chemosphere.2013.01.014. Epub 2013 Feb 10. Acute Effect Of Copper And Cadmium Exposure On The Expression Of Heat Shock Protein 70 In The Cyprinidae Fish...
Chen S, Zuo X, Yang M, Lu H, Wang N, Wang K, Tu Z, Chen G, Liu M, Liu K, Xiao X. J Leukoc Biol. 2012 Oct;92(4):851-7. Doi: 10.1189/Jlb.0212060. Epub 2012 Jun 29. Severe Multiple Organ Injury In Hsf1 Knockout Mice Induced By Lipopolysaccharide Is A...
Ouyang W, Gao F, Wang L, Xie X, Lei F, Zhou J, Liao Y, Zhong M, Tang J. Oncol Rep. 2010 Oct;24(4):973-9. Thermoseed Hyperthermia Treatment Of Mammary Orthotopic Transplantation Tumors In Rats And Impact On Immune Function.
Xu Xg, Luo Yj, Wu Y, Chen Jz, Xu Th, Gao Xh, He Cd, Geng L, Xiao T, Zhang Yq, Chen Hd, Li Yh. Dermatol Surg. 2011 Aug;37(8):1141-9. Doi: 10.1111/J.1524-4725.2011.02062.X. Epub 2011 Jun 7. Immunohistological Evaluation Of Skin Responses After Treat...
Chen N, Wan Xl, Huang Cx, Wang Wm, Liu H, Wang Hl. Immunobiology. 2014 Nov;219(11):850-8. Doi: 10.1016/J.Imbio.2014.07.012. Epub 2014 Jul 21. Study On The Immune Response To Recombinant Hsp70 Protein From Megalobrama Amblycephala.
Fan Sj, Jiang H, Yang Lj, Liu X, Song J, Pan F. Ann Anat. 2011 Oct 20;193(5):418-24. Doi: 10.1016/J.Aanat.2011.06.001. Epub 2011 Jul 8. Effects Of Adrenergic Agents On Stress-Induced Brain Microstructural And Immunochemical Changes In Adult Male W...
Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to hsp72 antibody, hspa1b antibody, hsp70i antibody