|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-HSP70 Antibody (Monoclonal, BRM-22)|
|Description||Mouse IgG monoclonal antibody for HSP70, heat shock 70kDa protein 1A; heat shock 70kDa protein 1B (HSPA1A; HSPA1B) detection. Tested with WB, IHC-P, IHC-F in Human;bovine;rabbit;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP70 Antibody (Monoclonal, BRM-22) BRM-22 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1050)|
|Replacement Item||This antibody may replace the following items: sc-33575|sc-59572 from Santa Cruz Biotechnology.|
|Validated Species||Bovine, Human, Rabbit, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||HSP70 isolated from bovine brain.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Heat shock 70 kDa protein 1A/1B|
|Molecular Weight||70176 MW|
|Protein Function||In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation. .|
|Sequence Similarities||Belongs to the heat shock protein 70 family.|
|Subcellular Localization||Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. .|
|Alternative Names||Heat shock 70 kDa protein 1B;Heat shock 70 kDa protein 1;HSP70.1;Hspa1b;Hcp70.1, Hsp70-1, Hsp70a1, Hspa1;|
|Research Areas||signal transduction|protein trafficking|chaperones|heat shock proteins|cancer|tumor biomarkers||
Background for Heat shock 70 kDa protein 1A/1B
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HSP70 Antibody (Monoclonal, BRM-22) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, By Heat
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Human, bovine, rabbit, rat, -
Western blot, 0.5μg/ml, Human, bovine, rabbit, rat
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.