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SKU:PA1813
Clonality:Polyclonal
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Hsp70 Antibody
Description Rabbit IgG polyclonal antibody for Heat shock 70 kDa protein 1A/1B(HSPA1A/HSPA1B) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Rat.
Cite This Product Anti-Hsp70 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1813)
Replacement Item This antibody may replace the following items: sc-33575|sc-59572 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Rat
Predicted Species Chicken

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70(577-596aa VISWLDANTLAEKDEFEHKR), different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name HSPA1A
Protein Name Heat shock 70 kDa protein 1A/1B
Molecular Weight 70052 MW
Protein Function In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Essential for STUB1-mediated ubiquitination and degradation of FOXP3 in regulatory T-cells (Treg) during inflammation (PubMed:23973223). .
Tissue Specificity HSPA1B is testis-specific.
Sequence Similarities Belongs to the heat shock protein 70 family.
Subcellular Localization Cytoplasm . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Uniprot ID P0DMV8
Alternative Names Heat shock 70 kDa protein 1A ;Heat shock 70 kDa protein 1;HSP70-1 ;HSP70.1;HSPA1A;HSPA1, HSX70;
Research Areas |signal transduction|protein trafficking|chaperones|heat shock proteins| cancer|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Heat shock 70 kDa protein 1A/1B

HSPA1(heat shock 70kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al.(1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Anti-Hsp70 Antibody Images

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Anti-Hsp70 Antibody
Anti-Hsp70 antibody, PA1813, Western blotting
All lanes: Anti Hsp70 (PA1813) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Predicted bind size: 70KD
Observed bind size: 70KD
Anti-Hsp70 Antibody
Anti-Hsp70 antibody, PA1813, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Hsp70 Antibody
Anti-Hsp70 antibody, PA1813, ICC
ICC: A549 Cell
Anti-Hsp70 Antibody
Anti-Hsp70 antibody, PA1813, IHC(F)
IHC(F):Rat Intestine Tissue
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Publications

Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X. Chemosphere. 2013 May;91(8):1113-22. Doi: 10.1016/J.Chemosphere.2013.01.014. Epub 2013 Feb 10. Acute Effect Of Copper And Cadmium Exposure On The Expression Of Heat Shock Protein 70 In The Cyprinidae Fish...
Chen S, Zuo X, Yang M, Lu H, Wang N, Wang K, Tu Z, Chen G, Liu M, Liu K, Xiao X. J Leukoc Biol. 2012 Oct;92(4):851-7. Doi: 10.1189/Jlb.0212060. Epub 2012 Jun 29. Severe Multiple Organ Injury In Hsf1 Knockout Mice Induced By Lipopolysaccharide Is A...
Ouyang W, Gao F, Wang L, Xie X, Lei F, Zhou J, Liao Y, Zhong M, Tang J. Oncol Rep. 2010 Oct;24(4):973-9. Thermoseed Hyperthermia Treatment Of Mammary Orthotopic Transplantation Tumors In Rats And Impact On Immune Function.
Xu Xg, Luo Yj, Wu Y, Chen Jz, Xu Th, Gao Xh, He Cd, Geng L, Xiao T, Zhang Yq, Chen Hd, Li Yh. Dermatol Surg. 2011 Aug;37(8):1141-9. Doi: 10.1111/J.1524-4725.2011.02062.X. Epub 2011 Jun 7. Immunohistological Evaluation Of Skin Responses After Treat...
Chen N, Wan Xl, Huang Cx, Wang Wm, Liu H, Wang Hl. Immunobiology. 2014 Nov;219(11):850-8. Doi: 10.1016/J.Imbio.2014.07.012. Epub 2014 Jul 21. Study On The Immune Response To Recombinant Hsp70 Protein From Megalobrama Amblycephala.
Fan Sj, Jiang H, Yang Lj, Liu X, Song J, Pan F. Ann Anat. 2011 Oct 20;193(5):418-24. Doi: 10.1016/J.Aanat.2011.06.001. Epub 2011 Jul 8. Effects Of Adrenergic Agents On Stress-Induced Brain Microstructural And Immunochemical Changes In Adult Male W...
Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to hsp72 antibody, hspa1b antibody, hsp70i antibody