Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™

HSP70/HSPA1A antibody

Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™ catalog # PB9638. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9638
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™

View all HSP70/HSPA1A Antibodies

SKU/Catalog Number

PB9638

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™ catalog # PB9638. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9638)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by five amino acids, and from the related rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9638 is reactive to HSPA1A in Human, Mouse, Rat

Applications

PB9638 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

70.052kDa

Background of HSP70/HSPA1A

HSPA1 (heat shock 70kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al. (1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For HSPA1A (Source: Uniprot.org, NCBI)

Gene Name

HSPA1A

Full Name

Heat shock 70 kDa protein 1A

Weight

70.052kDa

Superfamily

heat shock protein 70 family

Alternative Names

dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B HSPA1A HEL-S-103, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1 heat shock protein family A (Hsp70) member 1A heat shock 70 kDa protein 1A|HSP70-1/HSP70-2|HSP70.1/HSP70.2|Heat shock 70 kDa protein 1B|Heat shock 70 kDa protein 2|dnaK-type molecular chaperone HSP70-1|epididymis secretory protein Li 103|epididymis secretory sperm binding protein|heat shock 70 kDa protein 1|heat shock 70 kDa protein 1/2|heat shock 70 kDa protein 1A/1B|heat shock 70kD protein 1A|heat shock 70kDa protein 1A|heat shock-induced protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HSPA1A, check out the HSPA1A Infographic

HSPA1A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HSPA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9638 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Denatured corona proteins mediate the intracellular bioactivities of nanoparticles via the unfolded protein response
Species: Mouse

Pan W,Liang J,Tang H,Fang X,Wang F,Ding Y,Huang H,Zhang H.Differentially expressed microRNA profiles in exosomes from vascular smooth muscle cells associated with coronary artery calcification.Int J Biochem Cell Biol.2020 Jan;118:105645.doi:10.1016/j.bioc
Species: Mouse
PB9638 usage in article: APP:WB, SAMPLE:MOVAS-1 CELL, DILUTION:NA

Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system

Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.

Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats

Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

Zeng L, Xiao CZ, Deng ZT, Li RH. Evid Based Complement Alternat Med. 2017;2017:8985623. doi: 10.1155/2017/8985623. Epub 2017 Jan 11. Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model

Jing J, Liu H, Chen H, Hu S, Xiao K, Ma X. Chemosphere. 2013 May;91(8):1113-22. Doi: 10.1016/J.Chemosphere.2013.01.014. Epub 2013 Feb 10. Acute Effect Of Copper And Cadmium Exposure On The Expression Of Heat Shock Protein 70 In The Cyprinidae Fish...

Chen S, Zuo X, Yang M, Lu H, Wang N, Wang K, Tu Z, Chen G, Liu M, Liu K, Xiao X. J Leukoc Biol. 2012 Oct;92(4):851-7. Doi: 10.1189/Jlb.0212060. Epub 2012 Jun 29. Severe Multiple Organ Injury In Hsf1 Knockout Mice Induced By Lipopolysaccharide Is A...

Have you used Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

5 Customer Q&As for Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband™

Question

I was wanting to use using your anti-Hsp70/HSPA1A/HSPA1B antibody for regulation of cellular response to heat studies. Has this antibody been tested with western blotting on mouse liver tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-01-13

Answer

I appreciate your inquiry. This PB9638 anti-Hsp70/HSPA1A/HSPA1B antibody is tested on rat thymus tissue, liver tissue, tissue lysate, mouse liver tissue, kidney tissue, hela whole cell lysate, intestine tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-01-13

Question

We have seen staining in mouse colon carcinoma. Do you have any suggestions? Is anti-Hsp70/HSPA1A/HSPA1B antibody supposed to stain colon carcinoma positively?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

Based on literature colon carcinoma does express HSPA1A. Based on Uniprot.org, HSPA1A is expressed in endothelial cell, uterus, brain, muscle, pancreas, pns skin, embryonic kidney, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-29

Question

My colleagues were content with the WB result of your anti-Hsp70/HSPA1A/HSPA1B antibody. However we have been able to see positive staining in cajal-retzius cell fetal brain cortex cytoplasm. using this antibody. Is that expected? Could you tell me where is HSPA1A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

From what I have seen in literature, cajal-retzius cell fetal brain cortex does express HSPA1A. Generally HSPA1A expresses in cytoplasm. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-05-17

Question

We bought anti-Hsp70/HSPA1A/HSPA1B antibody for WB on embryonic kidney in the past. I am using mouse, and I plan to use the antibody for IHC next. I am interested in examining embryonic kidney as well as cajal-retzius cell fetal brain cortex in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

A. Thomas

Verified customer

Asked: 2018-10-30

Answer

I looked at the website and datasheets of our anti-Hsp70/HSPA1A/HSPA1B antibody and it seems that PB9638 has been validated on mouse in both WB and IHC. Thus PB9638 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-10-30

Question

We are currently using anti-Hsp70/HSPA1A/HSPA1B antibody PB9638 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?

G. Yang

Verified customer

Asked: 2014-09-05

Answer

The anti-Hsp70/HSPA1A/HSPA1B antibody (PB9638) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-09-05

Order DetailsPrice
PB9638

100μg

$370
PB9638-10ug

10μg sample (liquid)

$99
PB9638-Biotin

100 μg Biotin conjugated

$570
PB9638-Cy3

100 μg Cy3 conjugated

$570
PB9638-Dylight488

100 μg Dylight488 conjugated

$570
PB9638-Dylight550

100 μg Dylight550 conjugated

$570
PB9638-Dylight594

100 μg Dylight594 conjugated

$570
PB9638-FITC

100 μg FITC conjugated

$570
PB9638-HRP

100 μg HRP conjugated

$570
PB9638-APC

100 μg APC conjugated

$670
PB9638-PE

100 μg PE conjugated

$670
PB9638-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PB9638
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.