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SKU:MA1051
Pack Size:100μg/vial
Validated Species:Human, Mouse, Rabbit, Rat
Clonality:Monoclonal
Clone Number:AC-16
Host:Mouse
Application:WB
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Overview

Product Name Anti-HSP90 Antibody (Monoclonal, AC-16)
SKU/Catalog Number MA1051
Description Mouse IgG monoclonal antibody for HSP90, heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AA1;HSP90AB1) detection. Tested with WB in Human; mouse; rat; rabbit. No cross reactivity with other proteins.
Cite This Product Anti-HSP90 Antibody (Monoclonal, AC-16) AC-16 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1051)
Replacement Item This antibody may replace the following items: sc-160182|sc-160181 from Santa Cruz Biotechnology.
Host Mouse
Validated Species Human, Mouse, Rabbit, Rat
Predicted Species Bovine, Monkey

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
Immunogen Heat shock protein 90(HSP90) from the water mold Achlya ambisexualis.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number AC-16
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2b

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Hsp90ab1
Protein Name Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta
Molecular Weight 83281 MW
Protein Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). .
Subcellular Localization Cytoplasm . Melanosome .
Uniprot ID P34058
Alternative Names Heat shock protein HSP 90-beta;Heat shock 84 kDa;HSP 84;HSP84;Hsp90ab1;Hsp84, Hspcb;
Research Areas signal transduction|protein trafficking|chaperones|heat shock proteins|cancer|tumor biomarkers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta

Heat Shock Protein 90(HSP70) exists in multiple forms in mammalian cells. It has a unique 30-amino acid N terminus instead of the 223-amino acid ATP/geldanamycin-binding domain found at the N terminus of full-length HSPCA, which contains 732 amino acids. Functional proteomic screens reveal an essential extracellular role for hsp90-alpha in cancer cell invasiveness.
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Publications

Jiang Q, Wang Y, Li T, Shi K, Li Z, Ma Y, Li F, Luo H, Yang Y, Xu C. Mol Biol Cell. 2011 Apr 15;22(8):1167-80. Doi: 10.1091/Mbc.E10-10-0860. Epub 2011 Feb 23. Heat Shock Protein 90-Mediated Inactivation Of Nuclear Factor-??b Switches Autophagy To A...
Zhao Z, Miao Y, Pan P, Cheng B, Bai G, Wu H. Bmc Complement Altern Med. 2013 Aug 6;13:206. Doi: 10.1186/1472-6882-13-206. Qingfei Xiaoyan Wan Alleviates Asthma Through Multi-Target Network Regulation.

Customer Q&As

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to hsp90 antibody, hsp 90 antibody, hsp90 beta antibody