|Validated Species:||Human, Mouse, Rabbit, Rat|
Data & Images
|Product Name||Anti-HSP90 Antibody (Monoclonal, AC-16)|
|Description||Mouse IgG monoclonal antibody for HSP90, heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha (cytosolic), class B member 1 (HSP90AA1;HSP90AB1) detection. Tested with WB in Human; mouse; rat; rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-HSP90 Antibody (Monoclonal, AC-16) AC-16 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1051)|
|Replacement Item||This antibody may replace the following items: sc-160182|sc-160181 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rabbit, Rat|
|Predicted Species||Bovine, Monkey|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Heat shock protein 90(HSP90) from the water mold Achlya ambisexualis.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta|
|Molecular Weight||83281 MW|
|Protein Function||Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (By similarity). .|
|Subcellular Localization||Cytoplasm . Melanosome .|
|Alternative Names||Heat shock protein HSP 90-beta;Heat shock 84 kDa;HSP 84;HSP84;Hsp90ab1;Hsp84, Hspcb;|
|Research Areas||signal transduction|protein trafficking|chaperones|heat shock proteins|cancer|tumor biomarkers||
Background for Heat shock protein HSP 90-alpha; Heat shock protein HSP 90-beta
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-HSP90 Antibody (Monoclonal, AC-16) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 2-4μg/ml, Human, mouse, rabbit, rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.