Anti-human CD19 Monoclonal Antibody FITC Conjugated, Flow Validated

CD19 antibody

Boster Bio Anti-human CD19 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC00154-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC00154-FITC
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-human CD19 Monoclonal Antibody FITC Conjugated, Flow Validated

View all CD19 Antibodies

SKU/Catalog Number

FC00154-FITC

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD19 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC00154-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD19 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00154-FITC)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: 4G7

Isotype

IgG1,k

Immunogen

Human CCL (chronic lymphocytic leukemia) cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00154-FITC is reactive to CD19 in Human

Applications

FC00154-FITC is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

61.128kDa

Background of CD19

B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)

Gene Name

CD19

Full Name

B-lymphocyte antigen CD19

Weight

61.128kDa

Alternative Names

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12 CD19 B4, CVID3 CD19 molecule B-lymphocyte antigen CD19|B-lymphocyte surface antigen B4|T-cell surface antigen Leu-12|differentiation antigen CD19

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD19, check out the CD19 Infographic

CD19 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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10 Customer Q&As for Anti-human CD19 Monoclonal Antibody FITC Conjugated, Flow Validated

Question

Do you have a BSA free version of anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC available?

C. Thomas

Verified customer

Asked: 2020-01-21

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-21

Question

Will FC00154-FITC anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

You can see on the product datasheet, FC00154-FITC anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-30

Question

I see that the anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-16

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-16

Question

Can you help my question with product FC00154-FITC, anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-01-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00154-FITC anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-10

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-11-22

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-11-22

Question

Our lab want to know about to test anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC on human spleen for research purposes, then I may be interested in using anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Kulkarni

Verified customer

Asked: 2018-07-11

Answer

The products we sell, including anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-07-11

Question

Will anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC work for Flow Cytometry with spleen?

E. Jones

Verified customer

Asked: 2018-02-20

Answer

According to the expression profile of spleen, CD19 is highly expressed in spleen. So, it is likely that anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC will work for Flow Cytometry with spleen.

Boster Scientific Support

Answered: 2018-02-20

Question

See below the WB image, lot number and protocol we used for spleen using anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated FC00154-FITC. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-08-18

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-08-18

Question

Is a blocking peptide available for product anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated (FC00154-FITC)?

P. Wu

Verified customer

Asked: 2016-04-06

Answer

We do provide the blocking peptide for product anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated (FC00154-FITC). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2016-04-06

Question

I was wanting to use your anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?

D. Miller

Verified customer

Asked: 2014-12-15

Answer

It shows on the product datasheet, FC00154-FITC anti-human CD19 Monoclonal antibody FITC Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-12-15

Order DetailsPrice
FC00154-FITC-100tests

100 tests

$227
FC00154-FITC-200tests

200 tests

$353

Free Secondary Antibody

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FC00154-FITC
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$227.00

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