Anti-human CD2 Monoclonal Antibody FITC Conjugated, Flow Validated

CD2 antibody

Boster Bio Anti-human CD2 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC00570-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Product Info Summary

SKU: FC00570-FITC
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-human CD2 Monoclonal Antibody FITC Conjugated, Flow Validated

View all CD2 Antibodies

SKU/Catalog Number

FC00570-FITC

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD2 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC00570-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD2 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00570-FITC)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKT11

Isotype

IgG1,k

Immunogen

Human acute lymphoblastic leukemia cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00570-FITC is reactive to CD2 in Human

Applications

FC00570-FITC is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

39.448kDa

Background of CD2

CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al. (1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3 (LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD2 (Source: Uniprot.org, NCBI)

Gene Name

CD2

Full Name

T-cell surface antigen CD2

Weight

39.448kDa

Alternative Names

CD2 antigen (p50), sheep red blood cell receptor; CD2 antigen; CD2 molecule; CD2; Erythrocyte receptor; FLJ46032; LFA-2; LFA-3 receptor; lymphocyte-function antigen-2; Rosette receptor; SRBC; T11; T-cell surface antigen CD2; T-cell surface antigen T11/Leu-5 CD2 LFA-2, SRBC, T11 CD2 molecule T-cell surface antigen CD2|CD2 antigen (p50), sheep red blood cell receptor|LFA-3 receptor|T-cell surface antigen T11/Leu-5|erythrocyte receptor|lymphocyte-function antigen-2|rosette receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD2, check out the CD2 Infographic

CD2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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10 Customer Q&As for Anti-human CD2 Monoclonal Antibody FITC Conjugated, Flow Validated

Question

My lab would like to test anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC on human pancreas spleen for research purposes, then I may be interested in using anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

The products we sell, including anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-08

Question

I have a question about product FC00570-FITC, anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00570-FITC anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-29

Question

Would FC00570-FITC anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-10

Answer

You can see on the product datasheet, FC00570-FITC anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-10

Question

Is a blocking peptide available for product anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated (FC00570-FITC)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

We do provide the blocking peptide for product anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated (FC00570-FITC). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-04

Question

I was wanting to use your anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human pancreas spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pancreas spleen identification?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

As indicated on the product datasheet, FC00570-FITC anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-28

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas spleen using anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-27

Question

Will anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC work for Flow Cytometry with pancreas spleen?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

According to the expression profile of pancreas spleen, CD2 is highly expressed in pancreas spleen. So, it is likely that anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC will work for Flow Cytometry with pancreas spleen.

Boster Scientific Support

Answered: 2018-03-21

Question

Do you have a BSA free version of anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC available?

Verified Customer

Verified customer

Asked: 2017-08-07

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-07

Question

I see that the anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

V. Jones

Verified customer

Asked: 2016-06-15

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2016-06-15

Question

Here is the WB image, lot number and protocol we used for pancreas spleen using anti-human CD2 Monoclonal antibody FITC Conjugated, Flow Validated FC00570-FITC. Please let me know if you require anything else.

E. Bhatt

Verified customer

Asked: 2013-05-17

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-05-17

Order DetailsPrice
FC00570-FITC-100tests

100 tests

$235
FC00570-FITC-200tests

200 tests

$358

Free Secondary Antibody

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FC00570-FITC
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$235.00

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