Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated

Boster Bio Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated catalog # FC01572-Biotin. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: FC01572-Biotin
Size: 100µg
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated

See all Siglec-2/CD22 products

SKU/Catalog Number

FC01572-Biotin

Size

100µg

Form

Liquid

Description

Boster Bio Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated catalog # FC01572-Biotin. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at 4°C for one year. Do not freeze.

Cite This Product

Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01572-Biotin)

Host

Mouse

Contents

PBS with 0.09% NaN3

Clonality

Monoclonal

Clone Number

MYG13

Isotype

IgG1,k

Reactive Species

FC01572-Biotin is reactive to CD22 in Human

Applications

FC01572-Biotin is guaranteed for Flow Cytometry Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For CD22 (Source: Uniprot.Org, NCBI)

Uniprot ID

P20273

Gene ID

933

Gene Name

CD22

Full Name

B-cell receptor CD22

Weight

95.348kDa

Superfamily

immunoglobulin superfamily

Alternative Names

B-cell receptor CD22; BL-CAM; B-lymphocyte cell adhesion molecule; CD22 antigenMGC130020; CD22 molecule; CD22; sialic acid binding Ig-like lectin 2; Sialic acid-binding Ig-like lectin 2; Siglec2; Siglec-2; SIGLEC2FLJ22814; T-cell surface antigen Leu-14 CD22 SIGLEC-2, SIGLEC2 CD22 molecule B-cell receptor CD22|B-lymphocyte cell adhesion molecule|BL-CAM|CD22 antigen|T-cell surface antigen Leu-14|sialic acid-binding Ig-like lectin 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD22, check out the CD22 Infographic

CD22 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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10 Customer Q&As for Anti-human CD22/Siglec 2 Monoclonal Antibody Biotin Conjugated, Flow Validated

Question

Here is the WB image, lot number and protocol we used for spleen using anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-30

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-30

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-03

Question

Does FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-21

Answer

It shows on the product datasheet, FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-21

Question

Is a blocking peptide available for product anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01572-Biotin)?

Verified Customer

Verified customer

Asked: 2018-11-16

Answer

We do provide the blocking peptide for product anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated (FC01572-Biotin). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-11-16

Question

I see that the anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

R. Carter

Verified customer

Asked: 2018-01-04

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-01-04

Question

Does anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin work for Flow Cytometry with spleen?

D. Thomas

Verified customer

Asked: 2017-11-20

Answer

According to the expression profile of spleen, CD22 is highly expressed in spleen. So, it is likely that anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin will work for Flow Cytometry with spleen.

Boster Scientific Support

Answered: 2017-11-20

Question

I was wanting to use your anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated for Flow Cytometry for human spleen on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human spleen identification?

Verified Customer

Verified customer

Asked: 2017-07-11

Answer

It shows on the product datasheet, FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-11

Question

Is there a BSA free version of anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin available?

N. Jackson

Verified customer

Asked: 2017-06-06

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-06

Question

Can you help my question with product FC01572-Biotin, anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-05-31

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01572-Biotin anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-05-31

Question

My question regards to test anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin on human spleen for research purposes, then I may be interested in using anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

G. Wu

Verified customer

Asked: 2013-07-16

Answer

The products we sell, including anti-human CD22/Siglec 2 Monoclonal antibody Biotin Conjugated, Flow Validated FC01572-Biotin, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-07-16

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
FC01572-Biotin
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$224.00

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