Product Info Summary
SKU: | FC02675-PerCP-Cy5.5 |
---|---|
Size: | 100 tests, 200 tests |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
SKU/Catalog Number
FC02675-PerCP-Cy5.5
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC02675-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02675-PerCP-Cy5.5)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: OKT3
Isotype
IgG2a,k
Immunogen
Human peripheral blood lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
FC02675-PerCP-Cy5.5 is reactive to CD3E in Human
Applications
FC02675-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
23.147kDa
Background of CD3E
The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated PBMCs stained with anti-human CD3 PerCP Cyanine5.5 (clone OKT3, red histogram). PBMCs lymphocytes stained mouse IgG2a isotype control (blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD3E (Source: Uniprot.org, NCBI)
Gene Name
CD3E
Full Name
T-cell surface glycoprotein CD3 epsilon chain
Weight
23.147kDa
Alternative Names
CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD3E, check out the CD3E Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC02675-PerCP-Cy5.5)
Hello CJ!
FC02675-PerCP-Cy5.5 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis
Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.
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Customer Reviews
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
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10 Customer Q&As for Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated
Question
I am interested in to test anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 on human blood for research purposes, then I may be interested in using anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The products we sell, including anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-31
Question
Is a blocking peptide available for product anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC02675-PerCP-Cy5.5)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We do provide the blocking peptide for product anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC02675-PerCP-Cy5.5). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-20
Question
I have attached the WB image, lot number and protocol we used for blood using anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
Will FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
You can see on the product datasheet, FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-11
Question
I see that the anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
M. Walker
Verified customer
Asked: 2017-10-20
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-20
Question
Do you have a BSA free version of anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 available?
H. Taylor
Verified customer
Asked: 2016-08-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-08-18
Question
My question regarding product FC02675-PerCP-Cy5.5, anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Yang
Verified customer
Asked: 2015-09-16
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-09-16
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5. Let me know if you need anything else.
J. Krishna
Verified customer
Asked: 2015-08-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-08-26
Question
I was wanting to use your anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
C. Rodriguez
Verified customer
Asked: 2014-01-01
Answer
It shows on the product datasheet, FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-01-01
Question
Would anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 work for Flow Cytometry with blood?
D. Johnson
Verified customer
Asked: 2013-07-30
Answer
According to the expression profile of blood, CD3E is highly expressed in blood. So, it is likely that anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 will work for Flow Cytometry with blood.
Boster Scientific Support
Answered: 2013-07-30