Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

CD3E antibody

Boster Bio Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC02675-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: FC02675-PerCP-Cy5.5
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

View all CD3E Antibodies

SKU/Catalog Number

FC02675-PerCP-Cy5.5

Size

100 tests, 200 tests

Form

Liquid

Description

Boster Bio Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Catalog# FC02675-PerCP-Cy5.5). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02675-PerCP-Cy5.5)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: OKT3

Isotype

IgG2a,k

Immunogen

Human peripheral blood lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

FC02675-PerCP-Cy5.5 is reactive to CD3E in Human

Applications

FC02675-PerCP-Cy5.5 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

23.147kDa

Background of CD3E

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta, epsilon, and zeta. The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD3E (Source: Uniprot.org, NCBI)

Gene Name

CD3E

Full Name

T-cell surface glycoprotein CD3 epsilon chain

Weight

23.147kDa

Alternative Names

CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD3E, check out the CD3E Infographic

CD3E infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD3E: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

FC02675-PerCP-Cy5.5 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis

Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.

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10 Customer Q&As for Anti-human CD3 Cd3e Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

Question

I am interested in to test anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 on human blood for research purposes, then I may be interested in using anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The products we sell, including anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-31

Question

Is a blocking peptide available for product anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC02675-PerCP-Cy5.5)?

Verified Customer

Verified customer

Asked: 2019-08-20

Answer

We do provide the blocking peptide for product anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated (FC02675-PerCP-Cy5.5). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-20

Question

I have attached the WB image, lot number and protocol we used for blood using anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-26

Question

Will FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

You can see on the product datasheet, FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-02-11

Question

I see that the anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

M. Walker

Verified customer

Asked: 2017-10-20

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-10-20

Question

Do you have a BSA free version of anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 available?

H. Taylor

Verified customer

Asked: 2016-08-18

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-08-18

Question

My question regarding product FC02675-PerCP-Cy5.5, anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Yang

Verified customer

Asked: 2015-09-16

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-09-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5. Let me know if you need anything else.

J. Krishna

Verified customer

Asked: 2015-08-26

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-08-26

Question

I was wanting to use your anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?

C. Rodriguez

Verified customer

Asked: 2014-01-01

Answer

It shows on the product datasheet, FC02675-PerCP-Cy5.5 anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-01-01

Question

Would anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 work for Flow Cytometry with blood?

D. Johnson

Verified customer

Asked: 2013-07-30

Answer

According to the expression profile of blood, CD3E is highly expressed in blood. So, it is likely that anti-human CD3 Monoclonal antibody PerCP-Cy5.5 Conjugated, Flow Validated FC02675-PerCP-Cy5.5 will work for Flow Cytometry with blood.

Boster Scientific Support

Answered: 2013-07-30

Order DetailsPrice
FC02675-PerCP-Cy5.5-100tests

100 tests

$360
FC02675-PerCP-Cy5.5-200tests

200 tests

$612

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FC02675-PerCP-Cy5.5
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$360.00

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