Product Info Summary
SKU: | FC01513-FITC |
---|---|
Size: | 100 tests, 200 tests |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated
View all CD31/PECAM-1 Antibodies
SKU/Catalog Number
FC01513-FITC
Size
100 tests, 200 tests
Form
Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA
Description
Boster Bio Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated (Catalog# FC01513-FITC). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01513-FITC)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: JC70
Isotype
IgG1,k
Immunogen
Cell membrane preparation from the spleen of a patient with hairy cell leukemia
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01513-FITC is reactive to PECAM1 in Human
Applications
FC01513-FITC is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
82.522kDa
Background of CD31/PECAM-1
CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Monocytes gated PBMCs stained with FITC conjugated anti-human CD31 (clone JC70) (red histogram). Monocytes gated PBMCs stained with FITC conjugated mouse IgG1 isotype control (Blue histogram). The data are generated by BD Accuri C6 Flow Cytometer and analyzed by FlowJo software.
Protein Target Info & Infographic
Gene/Protein Information For PECAM1 (Source: Uniprot.org, NCBI)
Gene Name
PECAM1
Full Name
Platelet endothelial cell adhesion molecule
Weight
82.522kDa
Alternative Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 antigen|platelet endothelial cell adhesion molecule-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PECAM1, check out the PECAM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PECAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated (FC01513-FITC)
Hello CJ!
FC01513-FITC has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in vitro through activating ERK1/2 and AKT in a PAR1%u2011dependent manner
The induction of autophagy against mitochondria-mediated apoptosis in lung cancer cells by a ruthenium (II) imidazole complex
Wound Dressing Model of Human Umbilical Cord Mesenchymal Stem Cells-Alginates Complex Promotes Skin Wound Healing by Paracrine Signaling
Chen L, Chan Th, Yuan Yf, Hu L, Huang J, Ma S, Wang J, Dong Ss, Tang Kh, Xie D, Li Y, Guan Xy. J Clin Invest. 2010 Apr;120(4):1178-91. Doi: 10.1172/Jci40665. Epub 2010 Mar 24. Chd1L Promotes Hepatocellular Carcinoma Progression And Metastasis In M...
Qiu H, Yang B, Pei Zc, Zhang Z, Ding K. J Biol Chem. 2010 Oct 15;285(42):32638-46. Doi: 10.1074/Jbc.M110.105544. Epub 2010 Aug 2. Wss25 Inhibits Growth Of Xenografted Hepatocellular Cancer Cells In Nude Mice By Disrupting Angiogenesis Via Blocking...
Zhou S, Yi T, Liu R, Bian C, Qi X, He X, Wang K, Li J, Zhao X, Huang C, Wei Y. Mol Cell Proteomics. 2012 Jul;11(7):M112.017988. Doi: 10.1074/Mcp.M112.017988. Epub 2012 Apr 9. Proteomics Identification Of Annexin A2 As A Key Mediator In The Metasta...
Xiu J, Fan J, Li J, Cui G, Lei W. Biomed Res Int. 2014;2014:785146. Doi: 10.1155/2014/785146. Epub 2014 Jun 10. Different Angiogenic Abilities Of Self-Setting Calcium Phosphate Cement Scaffolds Consisting Of Different Proportions Of Fibrin Glue.
Wu Q, Chang Y, Zhang L, Zhang Y, Tian T, Feng G, Zhou S, Zheng Q, Han F, Huang F. J Cancer. 2013 Nov 21;4(9):727-35. Doi: 10.7150/Jca.7576. Ecollection 2013. Srpk1 Dissimilarly Impacts On The Growth, Metastasis, Chemosensitivity And Angiogenesis O...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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3 Customer Q&As for Anti-Human CD31 PECAM1 Monoclonal Antibody FITC Conjugated, Flow Validated
Question
Is this FC01513-FITC anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated reactive to the isotypes of PECAM1?
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
The immunogen of FC01513-FITC anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated is Cell membrane preparation from the spleen of a patient with hairy cell leukaemia. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-15
Question
We are interested in to test anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated FC01513-FITC on human tendon of biceps brachii for research purposes, then I may be interested in using anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated FC01513-FITC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
The products we sell, including anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated FC01513-FITC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-16
Question
I was wanting to use your anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated for Flow Cytometry for human tendon of biceps brachii on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tendon of biceps brachii identification?
C. Collins
Verified customer
Asked: 2017-06-16
Answer
You can see on the product datasheet, FC01513-FITC anti-Human CD31 Monoclonal antibody FITC Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human tendon of biceps brachii in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-16