Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated

PTPRC antibody

Boster Bio Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated (Catalog# FC00555-mFluor540). Tested in Flow Cytometry application(s). This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: FC00555-mFluor540
Size: 100 tests, 200 tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated

View all PTPRC Antibodies

SKU/Catalog Number

FC00555-mFluor540

Size

100 tests, 200 tests

Form

Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

Description

Boster Bio Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated (Catalog# FC00555-mFluor540). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00555-mFluor540)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: F10-89-4

Isotype

IgG2a,k

Immunogen

Human T lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00555-mFluor540 is reactive to PTPRC in Human

Applications

FC00555-mFluor540 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

147.486kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For PTPRC (Source: Uniprot.org, NCBI)

Gene Name

PTPRC

Full Name

Receptor-type tyrosine-protein phosphatase C

Weight

147.486kDa

Superfamily

protein-tyrosine phosphatase family

Alternative Names

B220; CD45 antigen; CD45; CD45R; EC 3.1.3.48; LCA; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; PTPRC; receptor-type tyrosine-protein phosphatase C; T200 Glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide PTPRC B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200 protein tyrosine phosphatase receptor type C receptor-type tyrosine-protein phosphatase C|CD45 antigen|T200 glycoprotein|T200 leukocyte common antigen|protein tyrosine phosphatase, receptor type, c polypeptide

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PTPRC, check out the PTPRC Infographic

PTPRC infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PTPRC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

FC00555-mFluor540 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis

Lipoxin A4 and its analogue suppress the tumor growth of transplanted H22 in mice: the role of antiangiogenesis

Detecting cell-in-cell structures in human tumor samples by E-cadherin/CD68/CD45 triple staining

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6 Customer Q&As for Anti-Human CD45 PTPRC Monoclonal Antibody mFluor540 Conjugated, Flow Validated

Question

I was wanting to use your anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated for Flow Cytometry for human erythroleukemia on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2020-05-04

Answer

As indicated on the product datasheet, FC00555-mFluor540 anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-05-04

Question

Is this FC00555-mFluor540 anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated reactive to the isotypes of PTPRC?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

The immunogen of FC00555-mFluor540 anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated is Human T lymphocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-30

Question

Does anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 work for Flow Cytometry with erythroleukemia?

Verified Customer

Verified customer

Asked: 2020-02-14

Answer

According to the expression profile of erythroleukemia, PTPRC is highly expressed in erythroleukemia. So, it is likely that anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 will work for Flow Cytometry with erythroleukemia.

Boster Scientific Support

Answered: 2020-02-14

Question

you antibody to test anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 on human erythroleukemia for research purposes, then I may be interested in using anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Huang

Verified customer

Asked: 2018-12-31

Answer

The products we sell, including anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-12-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2018-05-04

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-05-04

Question

I have attached the WB image, lot number and protocol we used for erythroleukemia using anti-Human CD45 Monoclonal antibody mFluor540 Conjugated, Flow Validated FC00555-mFluor540. Please let me know if you require anything else.

A. Li

Verified customer

Asked: 2013-11-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-11-12

Order DetailsPrice
FC00555-mFluor540-100tests

100 tests

$308
FC00555-mFluor540-200tests

200 tests

$511

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