Product Info Summary
SKU: | M00480-Dyl488 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2)
SKU/Catalog Number
M00480-Dyl488
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Human CD5 DyLight® 488 conjugated Antibody (monoclonal, 4E2) catalog # M00480-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480-Dyl488)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
4E2
Isotype
Mouse IgG1
Immunogen
E. coli-derived human CD5 recombinant protein (Position: R25-L495).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00480-Dyl488 is reactive to CD5 in Human
Applications
M00480-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
54.578kDa
Background of CD5
CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 1-3μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of A431 cells using anti-Human CD5 antibody (M00480-Dyl488).
Overlay histogram showing A431 cells stained with M00480-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human CD5 Antibody (M00480-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 2. Flow Cytometry analysis of HL-60 cells using anti-Human CD5 antibody (M00480-Dyl488).
Overlay histogram showing HL-60 cells stained with M00480-Dyl488 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Human CD5 Antibody (M00480-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)
Gene Name
CD5
Full Name
T-cell surface glycoprotein CD5
Weight
54.578kDa
Alternative Names
CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD5, check out the CD5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2) (M00480-Dyl488)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2)
Question
We have been able to see staining in human thymus. Any tips? Is anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) supposed to stain thymus positively?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
According to literature thymus does express CD5. According to Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-22
Question
My team were happy with the WB result of your anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2). However we have observed positive staining in thymus cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
From literature, thymus does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-01-08
Question
We are currently using anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) M00480-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) (M00480-Dyl488) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-07
Question
We are interested in using your anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) for cell recognition studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.
T. Kulkarni
Verified customer
Asked: 2015-01-26
Answer
I appreciate your inquiry. This M00480-Dyl488 anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) is tested on a431 cells. It is guaranteed to work for Flow Cytometry in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-01-26