Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2)

CD5 antibody

Boster Bio Anti-Human CD5 DyLight® 488 conjugated Antibody (monoclonal, 4E2) catalog # M00480-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00480-Dyl488
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2)

View all CD5 Antibodies

SKU/Catalog Number

M00480-Dyl488

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Human CD5 DyLight® 488 conjugated Antibody (monoclonal, 4E2) catalog # M00480-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00480-Dyl488)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

4E2

Isotype

Mouse IgG1

Immunogen

E. coli-derived human CD5 recombinant protein (Position: R25-L495).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00480-Dyl488 is reactive to CD5 in Human

Applications

M00480-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

54.578kDa

Background of CD5

CD5 is a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. In humans, the gene is located on the long arm of chromosome 11. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD5 (Source: Uniprot.org, NCBI)

Gene Name

CD5

Full Name

T-cell surface glycoprotein CD5

Weight

54.578kDa

Alternative Names

CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1 CD5 LEU1, T1 CD5 molecule T-cell surface glycoprotein CD5|CD5 antigen (p56-62)|epididymis secretory sperm binding protein|lymphocyte antigen T1/Leu-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD5, check out the CD5 Infographic

CD5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Human CD5 DyLight® 488 conjugated Antibody(monoclonal, 4E2)

Question

We have been able to see staining in human thymus. Any tips? Is anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) supposed to stain thymus positively?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

According to literature thymus does express CD5. According to Uniprot.org, CD5 is expressed in leukocyte, lymphocyte, thymus, pancreas, leukemic t-cell, among other tissues. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-22

Question

My team were happy with the WB result of your anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2). However we have observed positive staining in thymus cell membrane using this antibody. Is that expected? Could you tell me where is CD5 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

From literature, thymus does express CD5. Generally CD5 expresses in cell membrane. Regarding which tissues have CD5 expression, here are a few articles citing expression in various tissues:
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Lymphocyte, Pubmed ID: 8740779
Pancreas, Pubmed ID: 15489334
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-01-08

Question

We are currently using anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) M00480-Dyl488 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

The anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) (M00480-Dyl488) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-07

Question

We are interested in using your anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) for cell recognition studies. Has this antibody been tested with western blotting on a431 cells? We would like to see some validation images before ordering.

T. Kulkarni

Verified customer

Asked: 2015-01-26

Answer

I appreciate your inquiry. This M00480-Dyl488 anti-Human CD5 DyLight® 488 conjugated antibody(monoclonal, 4E2) is tested on a431 cells. It is guaranteed to work for Flow Cytometry in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2015-01-26

Order DetailsPrice
M00480-Dyl488

100μg

$515
M00480-Dyl488-carrier-free

Carrier Free

$515

Free Secondary Antibody

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M00480-Dyl488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$515.00

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