Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated

CD8 antibody

Boster Bio Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated catalog # FC02236. Tested in Flow Cytometry applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: FC02236
Size: 100ug, 500ug
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated

View all CD8 Antibodies

SKU/Catalog Number

FC02236

Size

100ug, 500ug

Form

Liquid

Description

Boster Bio Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated catalog # FC02236. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at 4°C for one year. Do not freeze.

Cite This Product

Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC02236)

Host

Mouse

Contents

PBS, pH7.2, 0.09% NaN3

Clonality

Monoclonal

Clone Number

OKT8

Isotype

IgG2a,k

Reactive Species

FC02236 is reactive to Cd8a in Human

Applications

FC02236 is guaranteed for Flow Cytometry Boster Guarantee

Background of CD8

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For Cd8a (Source: Uniprot.org, NCBI)

Uniprot ID

P01731

Gene ID

12525

Gene Name

Cd8a

Full Name

T-cell surface glycoprotein CD8 alpha chain

Weight

27.456kDa

Alternative Names

53-6.7 CD8 Alpha; 53-6.7 CD8 Aplha; 53-6.7 CD8; 53-6.7 Clone; 53-6.7; CD8; CD8; CD8a molecule; CD8A; Cytotoxic T cell marker; Cytotoxic T lymphocyte marker; LEU2; p32; T cell marker Cd8a|BB154331, Ly-, Ly-2, Ly-3, Ly-35, Ly-B, Lyt-, Lyt-2|CD8 antigen, alpha chain|T-cell surface glycoprotein CD8 alpha chain|Lyt-2.1 lymphocyte differentiation antigen (AA at 100)|T-cell surface glycoprotein Lyt-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Cd8a, check out the Cd8a Infographic

Cd8a infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Cd8a: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

FC02236 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients

Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...

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10 Customer Q&As for Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated

Question

I was wanting to use your anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated for Flow Cytometry for human pericardium on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pericardium identification?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

As indicated on the product datasheet, FC02236 anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human pericardium in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-22

Question

See below the WB image, lot number and protocol we used for pericardium using anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-02

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pericardium using anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-12

Question

I am interested in to test anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 on human pericardium for research purposes, then I may be interested in using anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

D. Banerjee

Verified customer

Asked: 2019-12-09

Answer

The products we sell, including anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-09

Question

Would FC02236 anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

You can see on the product datasheet, FC02236 anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-19

Question

I see that the anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-17

Question

My question regarding product FC02236, anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-02-23

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC02236 anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-02-23

Question

Would anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 work for Flow Cytometry with pericardium?

M. Yang

Verified customer

Asked: 2016-02-10

Answer

According to the expression profile of pericardium, CD8A is highly expressed in pericardium. So, it is likely that anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 will work for Flow Cytometry with pericardium.

Boster Scientific Support

Answered: 2016-02-10

Question

Do you have a BSA free version of anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 available?

W. Kulkarni

Verified customer

Asked: 2014-02-24

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated FC02236 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2014-02-24

Question

Is a blocking peptide available for product anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated (FC02236)?

D. Jackson

Verified customer

Asked: 2013-09-25

Answer

We do provide the blocking peptide for product anti-human CD8 Monoclonal antibody Unconjugated, Flow Validated (FC02236). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-09-25

Size

Total: $114

SKU:FC02236

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
FC02236
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$114.00

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