|Application:||ELISA, IHC-P, WB|
Data & Images
|Product Name||Anti-FGF1/Fgf Acidic Antibody|
|Description||Rabbit IgG polyclonal antibody for Fibroblast growth factor 1(FGF1) detection. Tested with WB, IHC-P, ELISA in Human.|
|Cite This Product||Anti-FGF1/Fgf Acidic Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1005)|
|Replacement Item||This antibody may replace the following items: sc-13123|sc-1359|sc-1884|sc-4572|sc-55520|sc-7910 from Santa Cruz Biotechnology.|
|Application||ELISA, IHC-P, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E. coli-derived human FGF1 recombinant protein(Position: F16-D155).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Fibroblast growth factor 1|
|Molecular Weight||17460 MW|
|Protein Function||Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. .|
|Tissue Specificity||Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .|
|Sequence Similarities||Belongs to the heparin-binding growth factors family.|
|Subcellular Localization||Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59. .|
|Alternative Names||Fibroblast growth factor 1;FGF-1;Acidic fibroblast growth factor;aFGF;Endothelial cell growth factor;ECGF;Heparin-binding growth factor 1;HBGF-1;FGF1;FGFA;|
|Research Areas|||cardiovascular|angiogenesis|growth factors|fgf| signal transduction|growth factors/hormones| cancer||
Background for Fibroblast growth factor 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-FGF1/Fgf Acidic Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-FGF1/Fgf Acidic Antibody Images
Click the images to enlarge.
Lane 1: Recombinant Human FGF1 Protein 10ng
Lane 2: Recombinant Human FGF1 Protein 5ng
Lane 3: Recombinant Human FGF1 Protein 2
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,