Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated

CD74 antibody

Boster Bio Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (Catalog# FC01340-PE-Cy7). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.

Product Info Summary

SKU: FC01340-PE-Cy7
Size: 100 tests, 200 tests
Reactive Species: Cynomolgus monkey, Human, Baboon, Rhesus monkey
Host: Mouse
Application: Flow Cytometry

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Product Name

Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated

View all CD74 Antibodies

SKU/Catalog Number

FC01340-PE-Cy7

Size

100 tests, 200 tests

Form

Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

Description

Boster Bio Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (Catalog# FC01340-PE-Cy7). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01340-PE-Cy7)

Host

Mouse

Contents

PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: L243

Isotype

IgG2a,k

Immunogen

Human B lymphocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC01340-PE-Cy7 is reactive to CD74 in Cynomolgus monkey, Human, Baboon, Rhesus monkey

Applications

FC01340-PE-Cy7 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

33.516kDa

Background of CD74

HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kD α (heavy) chain and a 27 kD β (light) chain. It is expressed on B cells, activated T cells, monocytes/macrophages, dendritic cells, and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

Gene/Protein Information For CD74 (Source: Uniprot.org, NCBI)

Gene Name

CD74

Full Name

HLA class II histocompatibility antigen gamma chain

Weight

33.516kDa

Alternative Names

CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated); CD74 antigen; CD74 molecule, major histocompatibility complex, class II invariant chain; CD74; CLIP; DHLAG; DHLAGgamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLADG; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ia-gamma; Ii; INVG34; MHC HLA-DR gamma chain; p33 CD74 DHLAG, HLADG, II, Ia-GAMMA, p33 CD74 molecule HLA class II histocompatibility antigen gamma chain|CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)|CD74 molecule, major histocompatibility complex, class II invariant chain|HLA-DR antigens-associated invariant chain|HLA-DR-gamma|Ia-associated invariant chain|MHC HLA-DR gamma chain|gamma chain of class II antigens

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD74, check out the CD74 Infographic

CD74 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD74: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Human HLA-DR CD74 Monoclonal Antibody PE-Cy7 Conjugated, Flow Validated

Question

Do you have a BSA free version of anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated FC01340-PE-Cy7 available?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated FC01340-PE-Cy7 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-17

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell tonsil using anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated FC01340-PE-Cy7. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-15

Question

My question regarding product FC01340-PE-Cy7, anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01340-PE-Cy7 anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-10

Question

I was wanting to use your anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated for Flow Cytometry for human b-cell tonsil on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human b-cell tonsil identification?

Verified Customer

Verified customer

Asked: 2019-06-26

Answer

It shows on the product datasheet, FC01340-PE-Cy7 anti-Human HLA-DR Monoclonal antibody PE-Cy7 Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell tonsil in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-26

Order DetailsPrice
FC01340-PE-Cy7-100tests

100 tests

$338
FC01340-PE-Cy7-200tests

200 tests

$561

Free Secondary Antibody

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FC01340-PE-Cy7
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$338.00

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