Anti-IFN gamma Antibody

IFN-gamma antibody

Boster Bio Anti-IFN gamma Antibody catalog # RP1002. Tested in IHC, WB applications. This antibody reacts with Human. Cited in 5 publication(s).

Product Info Summary

SKU: RP1002
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-IFN gamma Antibody

View all IFN-gamma Antibodies

SKU/Catalog Number

RP1002

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-IFN gamma Antibody catalog # RP1002. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-IFN gamma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1002)

Host

Rabbit

Contents

Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Carrier free (No BSA) form available in stock. If you want this antibody carrier free please specify "Carrier Free" or "No BSA" in your order note.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human IFN gamma recombinant protein (Position: Q24-Q166).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1002 is reactive to IFNG in Human

Applications

RP1002 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

19.348kDa

Background of IFN-gamma

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For IFNG (Source: Uniprot.org, NCBI)

Gene Name

IFNG

Full Name

Interferon gamma

Weight

19.348kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma IFNG IFG, IFI, IMD69 interferon gamma interferon gamma|IFN-gamma|immune interferon

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IFNG, check out the IFNG Infographic

IFNG infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IFNG: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

RP1002 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1?-Hydroxylase by the Fetal-Maternal Interface

Protective effect of carnosine after chronic cerebral hypoperfusion possibly through suppressing astrocyte activation

Somatostatin Improved B Cells Mature in Macaques during Intestinal Ischemia-Reperfusion

Somatostatin Inhibits the Production of Interferon-? by Intestinal Epithelial Cells During Intestinal Ischemia?Reperfusion in Macaques

Yan X, Wang D, Liang F, Fu L, Guo C. Hum Vaccin Immunother. 2014;10(12):3491-8. Doi: 10.4161/Hv.36084. Hpv16L1-Attenuated Shigella Recombinant Vaccine Induced Strong Vaginal And Systemic Immune Responses In Guinea Pig Model.

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4 Customer Q&As for Anti-IFN gamma Antibody

Question

My team were well pleased with the WB result of your anti-IFN gamma antibody. However we have seen positive staining in leukocyte secreted. using this antibody. Is that expected? Could you tell me where is IFNG supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

According to literature, leukocyte does express IFNG. Generally IFNG expresses in secreted. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-06-11

Question

We have seen staining in human blood. What should we do? Is anti-IFN gamma antibody supposed to stain blood positively?

R. Jha

Verified customer

Asked: 2017-03-08

Answer

From what I have seen in literature blood does express IFNG. From what I have seen in Uniprot.org, IFNG is expressed in leukocyte, blood, among other tissues. Regarding which tissues have IFNG expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2017-03-08

Question

We are currently using anti-IFN gamma antibody RP1002 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?

S. Krishna

Verified customer

Asked: 2016-06-17

Answer

The anti-IFN gamma antibody (RP1002) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-06-17

Question

We purchased anti-IFN gamma antibody for WB on leukocyte in a previous experiment. I am using human, and We want to use the antibody for IHC next. I would like examining leukocyte as well as blood in our next experiment. Do you have any suggestion on which antibody would work the best for IHC?

S. Jones

Verified customer

Asked: 2013-07-15

Answer

I looked at the website and datasheets of our anti-IFN gamma antibody and it appears that RP1002 has been validated on human in both WB and IHC. Thus RP1002 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2013-07-15

Order DetailsPrice
RP1002

100μg

$370
RP1002-10ug

10μg sample (liquid)

$99
RP1002-Biotin

100 μg Biotin conjugated

$570
RP1002-Cy3

100 μg Cy3 conjugated

$570
RP1002-Dylight488

100 μg Dylight488 conjugated

$570
RP1002-Dylight550

100 μg Dylight550 conjugated

$570
RP1002-Dylight594

100 μg Dylight594 conjugated

$570
RP1002-FITC

100 μg FITC conjugated

$570
RP1002-HRP

100 μg HRP conjugated

$570
RP1002-APC

100 μg APC conjugated

$670
RP1002-PE

100 μg PE conjugated

$670
RP1002-carrier-free

Carrier Free

$370

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