Anti-Human IL22 DyLight® 488 conjugated Antibody(monoclonal, 7F2)

Boster Bio Anti-Human IL22 DyLight® 488 conjugated Antibody (monoclonal, 7F2) catalog # M00963-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M00963-Dyl488
Size: 50ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-Human IL22 DyLight® 488 conjugated Antibody(monoclonal, 7F2)

See all IL-22 products

SKU/Catalog Number

M00963-Dyl488

Size

50ug/vial

Form

Liquid

Description

Boster Bio Anti-Human IL22 DyLight® 488 conjugated Antibody (monoclonal, 7F2) catalog # M00963-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At 2-8?C for one year from date of receipt. Protect from light. Do not freeze.

Cite This Product

Anti-Human IL22 DyLight® 488 conjugated Antibody(monoclonal, 7F2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00963-Dyl488)

Host

Mouse

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Monoclonal

Clone Number

7F2

Isotype

IgG1

Immunogen

E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

M00963-Dyl488 is reactive to IL22 in Human

Applications

M00963-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For IL22 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9GZX6

Gene ID

50616

Gene Name

IL22

Full Name

Interleukin-22

Weight

20.011kDa

Superfamily

IL-10 family

Alternative Names

Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL22; IL-22; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18 IL22 IL-21, IL-22, IL-D110, IL-TIF, ILTIF, TIFIL-23, TIFa, zcyto18 interleukin 22 interleukin-22|IL-10-related T-cell-derived inducible factor|cytokine Zcyto18

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL22, check out the IL22 Infographic

IL22 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL22: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for M00963-Dyl488

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Human IL22 DyLight® 488 conjugated Antibody(monoclonal, 7F2)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Human IL22 DyLight® 488 conjugated Antibody(monoclonal, 7F2)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

14 Customer Q&As for Anti-Human IL22 DyLight® 488 conjugated Antibody(monoclonal, 7F2)

Question

My question regarding product M00963-Dyl488, anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00963-Dyl488 anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-01-31

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488. Let me know if you need anything else.

C. Miller

Verified customer

Asked: 2020-01-21

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-01-21

Question

My colleagues were well pleased with the WB result of your anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2). However we have observed positive staining in zone of skin secreted. using this antibody. Is that expected? Could you tell me where is IL22 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

Based on literature, zone of skin does express IL22. Generally IL22 expresses in secreted. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-18

Question

Is there a BSA free version of anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 available?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-11

Question

Is this M00963-Dyl488 anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) reactive to the isotypes of IL22?

Verified Customer

Verified customer

Asked: 2019-10-07

Answer

The immunogen of M00963-Dyl488 anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) is E. coli-derived human IL-22 recombinant protein (Position: A34-I179). Human IL-22 shares 81.4% amino acid (aa) sequence identity with mouse IL-22. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-10-07

Question

Is a blocking peptide available for product anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) (M00963-Dyl488)?

Verified Customer

Verified customer

Asked: 2019-03-27

Answer

We do provide the blocking peptide for product anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) (M00963-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-03-27

Question

you antibody to test anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 on human blood for research purposes, then I may be interested in using anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-01-22

Answer

The products we sell, including anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-01-22

Question

We have been able to see staining in human zone of skin. Any tips? Is anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) supposed to stain zone of skin positively?

Verified Customer

Verified customer

Asked: 2018-01-24

Answer

According to literature zone of skin does express IL22. According to Uniprot.org, IL22 is expressed in zone of skin, blood, among other tissues. Regarding which tissues have IL22 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-01-24

Question

I see that the anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-12-04

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-12-04

Question

We are currently using anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-09

Answer

The anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) (M00963-Dyl488) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-09

Question

Does anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 work for Flow Cytometry with blood?

O. Mangal

Verified customer

Asked: 2017-06-09

Answer

According to the expression profile of blood, IL22 is highly expressed in blood. So, it is likely that anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488 will work for Flow Cytometry with blood.

Boster Scientific Support

Answered: 2017-06-09

Question

I was wanting to use your anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human blood identification?

M. Jones

Verified customer

Asked: 2015-07-27

Answer

You can see on the product datasheet, M00963-Dyl488 anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-07-27

Question

Does M00963-Dyl488 anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

K. Miller

Verified customer

Asked: 2014-03-25

Answer

As indicated on the product datasheet, M00963-Dyl488 anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-03-25

Question

Please see the WB image, lot number and protocol we used for blood using anti-Human IL22 DyLight® 488 conjugated antibody(monoclonal, 7F2) M00963-Dyl488. Please let me know if you require anything else.

K. Anderson

Verified customer

Asked: 2013-12-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-12-10

$290

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
M00963-Dyl488
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$290.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.