|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-MFGE8/Lactadherin Antibody|
|Description||Rabbit IgG polyclonal antibody for Lactadherin(MFGE8) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-MFGE8/Lactadherin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1945)|
|Replacement Item||This antibody may replace the following items: sc-23999|sc-271574|sc-33545|sc-56564|sc-71573|sc-71574|sc-8029 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Human Milk Fat Globule 1(165-180aa NGHEFDFIHDVNKKHK).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||43123 MW|
|Protein Function||Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication. .|
|Tissue Specificity||Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.|
|Sequence Similarities||Contains 1 EGF-like domain.|
|Subcellular Localization||Membrane ; Peripheral membrane protein . Secreted .|
|Alternative Names||Lactadherin;Breast epithelial antigen BA46;HMFG;MFGM;Milk fat globule-EGF factor 8;MFG-E8;SED1;Lactadherin short form;Medin;MFGE8;|
|Research Areas|||cell biology|apoptosis|phagocytosis| cardiovascular|angiogenesis|angiogenic factors| kits/ lysates/ other|elisa kits|adhesion molecules elisa kits|apoptosis marker and proteins elisa kits|angiogenic factors elisa kits| metabolism|pathways and processes|metabolism processes| cancer|cell death||
Background for Lactadherin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-MFGE8/Lactadherin Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-MFGE8/Lactadherin Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant human MEGF8, 50.2KD (162aa tag+ S28-A317)
Lane 1: Recombinant Human MEGF8 Protein 10ng
Lane 2: Recombinant Human MEGF8 Protein 5ng
Lane 3: Recombinant Human MEGF8 Protein 2.5ng
Lane 4: Recombinant Human MEGF8 Protein 1.25ng
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,