Anti-Human NFAT2 DyLight® 488 conjugated NFATC1 Antibody

NFATC1/NFAT2 antibody

Boster Bio Anti-Human NFAT2 DyLight® 488 conjugated NFATC1 Antibody catalog # A00340-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: A00340-Dyl488
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry

Product Name

Anti-Human NFAT2 DyLight® 488 conjugated NFATC1 Antibody

View all NFATC1/NFAT2 Antibodies

SKU/Catalog Number

A00340-Dyl488

Size

100 μg/vial

Form

Liquid

Description

Boster Bio Anti-Human NFAT2 DyLight® 488 conjugated NFATC1 Antibody catalog # A00340-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Cite This Product

Anti-Human NFAT2 DyLight® 488 conjugated NFATC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00340-Dyl488)

Host

Rabbit

Contents

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00340-Dyl488 is reactive to NFATC1 in Human

Applications

A00340-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee

Background of NFATC1/NFAT2

NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1), also known as NFATC or NFAT2, is a protein that in humans is encoded by the NFATC1 gene. And it is also a component of the nuclear factor of activated T cells DNA-binding transcription complex. The NFATC1 gene is mapped on 18q23. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. NFATC1 was not detected in brain, liver, or kidney. NFATC1 is an inducible nuclear component which functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. The solution structure of the binary complex formed between the core DNA-binding domain of human NFATC1 and the 12-bp oligonucleotide duplex containing the ARRE2 DNA site from the IL2 promoter. Nfatc1 was expressed exclusively in mouse hair follicle stem cells, and using gain- and loss-of-function approaches, and Nfatc1 inhibited stem cell activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For NFATC1 (Source: Uniprot.org, NCBI)

Uniprot ID

O95644

Gene ID

4772

Gene Name

NFATC1

Full Name

Nuclear factor of activated T-cells, cytoplasmic 1

Weight

101.243kDa

Alternative Names

MGC138448; NFAT transcription complex cytosolic component; NFAT2; NFAT2cytoplasmic 1; NFATC; NF-ATC; NFATC1; NF-ATc1; nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 NFATC1 NF-ATC, NF-ATc1.2, NFAT2, NFATc nuclear factor of activated T cells 1 nuclear factor of activated T-cells, cytoplasmic 1|NFAT transcription complex cytosolic component|nuclear factor of activated T-cells c|nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on NFATC1, check out the NFATC1 Infographic

NFATC1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for NFATC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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14 Customer Q&As for Anti-Human NFAT2 DyLight® 488 conjugated NFATC1 Antibody

Question

Will A00340-Dyl488 anti-Human NFAT2 DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

It shows on the product datasheet, A00340-Dyl488 anti-Human NFAT2 DyLight® 488 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-05-01

Question

Is this A00340-Dyl488 anti-Human NFAT2 DyLight® 488 conjugated antibody reactive to the isotypes of NFATC1?

A. Mitchell

Verified customer

Asked: 2020-03-19

Answer

The immunogen of A00340-Dyl488 anti-Human NFAT2 DyLight® 488 conjugated antibody is E. coli-derived human NFAT2 recombinant protein (Position: Q589-K652). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-03-19

Question

Is there a BSA free version of anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 available?

Verified Customer

Verified customer

Asked: 2020-03-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-17

Question

See attached the WB image, lot number and protocol we used for b-cell using anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-25

Question

Would anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 work for Flow Cytometry with b-cell?

Verified Customer

Verified customer

Asked: 2019-12-11

Answer

According to the expression profile of b-cell, NFATC1 is highly expressed in b-cell. So, it is likely that anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 will work for Flow Cytometry with b-cell.

Boster Scientific Support

Answered: 2019-12-11

Question

I see that the anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-04

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-04

Question

We have observed staining in human b-cell. Any tips? Is anti-Human NFAT2 DyLight® 488 conjugated antibody supposed to stain b-cell positively?

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

Based on literature b-cell does express NFATC1. Based on Uniprot.org, NFATC1 is expressed in muscle organ, peripheral blood lymphocyte t-cell, b-cell, b-cell lymphoma, brain, erythroleukemia, among other tissues. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141

Boster Scientific Support

Answered: 2019-10-01

Question

Our lab were well pleased with the WB result of your anti-Human NFAT2 DyLight® 488 conjugated antibody. However we have seen positive staining in erythroleukemia cytoplasm. using this antibody. Is that expected? Could you tell me where is NFATC1 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

From what I have seen in literature, erythroleukemia does express NFATC1. Generally NFATC1 expresses in cytoplasm. Regarding which tissues have NFATC1 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8702849
B-cell lymphoma, Pubmed ID: 10072078
Brain, Pubmed ID: 15489334
Erythroleukemia, Pubmed ID: 23186163
Peripheral blood lymphocyte, and T-cell, Pubmed ID: 8202141

Boster Scientific Support

Answered: 2019-08-12

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell using anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-05-13

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-13

Question

My question regards to test anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 on human b-cell for research purposes, then I may be interested in using anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-02-06

Answer

The products we sell, including anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-02-06

Question

We are currently using anti-Human NFAT2 DyLight® 488 conjugated antibody A00340-Dyl488 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

The anti-Human NFAT2 DyLight® 488 conjugated antibody (A00340-Dyl488) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-03-19

Question

Is a blocking peptide available for product anti-Human NFAT2 DyLight® 488 conjugated antibody (A00340-Dyl488)?

Verified Customer

Verified customer

Asked: 2017-05-18

Answer

We do provide the blocking peptide for product anti-Human NFAT2 DyLight® 488 conjugated antibody (A00340-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-05-18

Question

My question regarding product A00340-Dyl488, anti-Human NFAT2 DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

H. Zhang

Verified customer

Asked: 2016-09-22

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00340-Dyl488 anti-Human NFAT2 DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-09-22

Question

I was wanting to use your anti-Human NFAT2 DyLight® 488 conjugated antibody for Flow Cytometry for human b-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human b-cell identification?

E. Li

Verified customer

Asked: 2013-06-13

Answer

It shows on the product datasheet, A00340-Dyl488 anti-Human NFAT2 DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-06-13

Size

Total: $515

SKU:A00340-Dyl488

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