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SKU:RP1022
Clonality:Polyclonal
Application:ELISA, IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-human Transferrin Antibody
Description Rabbit IgG polyclonal antibody for Serotransferrin(TF) detection. Tested with WB, IHC-P, ELISA in Human.
Cite This Product Anti-human Transferrin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1022)
Replacement Item This antibody may replace the following items: sc-80952|sc-23596|sc-20160|sc-30201|sc-374441|sc-59714|sc-393657|sc-18786|sc-376361 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application ELISA, IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Oryza sativa-derived human Transferrin recombinant protein(Position: V20-P698).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name TF
Protein Name Serotransferrin(Transferrin)
Molecular Weight 77064 MW
Protein Function Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Tissue Specificity Expressed by the liver and secreted in plasma.
Sequence Similarities Belongs to the transferrin family.
Subcellular Localization Secreted.
Uniprot ID P02787
Alternative Names Serotransferrin;Transferrin;Beta-1 metal-binding globulin;Siderophilin;TF;PRO1400;
Research Areas |tags & cell markers|cell type markers|tumor associated| epigenetics and nuclear signaling|transcription|domain families|hlh / leucine zipper|transcription factors|chip'ing antibodies|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Serotransferrin(Transferrin)

Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. In humans, it is encoded by the TF gene. In humans, transferrin consists of a polypeptide chain containing 679 amino acids. The protein is composed of alpha helices and beta sheets to form two domains. The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1%(4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover(25 mg/24 h). Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites. The affinity of transferrin for Fe(III) is extremely high(1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.

Anti-human Transferrin Antibody Images

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Anti-human Transferrin Antibody
Figure. Western blot analysis of Transferrin using anti- Transferrin antibody (RP1022).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane : Recombinant Human Transferrin Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Transferrin antigen affinity purified polyclonal antibody (Catalog # RP1022) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Transferrin at approximately 76KD. The expected band size for Transferrin is at 76KD.
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Publications

Chen L, Luo G, Tan Y, Wei J, Wu C, Zheng L, Zhang X, Xu N. Acta Histochem. 2010 May;112(3):233-9. Doi: 10.1016/J.Acthis.2008.11.024. Epub 2009 May 9. Immunolocalisation Of Tissue Factor In Esophageal Cancer Is Correlated With Intratumoral Angiogen...
Chen K, Li Z, Jiang P, Zhang X, Zhang Y, Jiang Y, He Y, Li X. Oncol Rep. 2014 Aug;32(2):755-63. Doi: 10.3892/Or.2014.3245. Epub 2014 Jun 11. Co-Expression Of Cd133, Cd44V6 And Human Tissue Factor Is Associated With Metastasis And Poor Prognosis In...
Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...
Wang G, Hu W, Tang Q, Wang L, Sun Xg, Chen Y, Yin Y, Xue F, Sun Z. Mol Neurobiol. 2015 Jun 23. [Epub Ahead Of Print] Effect Comparison Of Both Iron Chelators On Outcomes, Iron Deposit, And Iron Transporters After Intracerebral Hemorrhage In Rats.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to cd71 antibody, tr antibody, tfr antibody