Anti-Huntington HTT Antibody

Huntingtin antibody

Boster Bio Anti-Huntington HTT Antibody (Catalog # A00134). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A00134
Size: 100ug
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-Huntington HTT Antibody

View all Huntingtin Antibodies

SKU/Catalog Number

A00134

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Huntington HTT Antibody (Catalog # A00134). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is six (6) months from date of opening.

Cite This Product

Anti-Huntington HTT Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00134)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol

Clonality

Polyclonal

Isotype

IgG

Immunogen

Huntington affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the near N-terminus of human Huntington disease protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A00134 is reactive to HTT in Human, Mouse, Rat

Applications

A00134 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

347.603kDa

Background of Huntingtin

Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization. This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation. Anti-Huntington antibodies are ideal for researchers interested in Apoptosis, Autophagy, Cytoskeleton, Neurodegeneration, Neuroscience, and Neuronal Cell Markers research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000 - 1:60,000
IHC: 1:100-1:500
IF Microscopy: 1:100-1:500
WB: 0.1 µg/ml

Validation Images & Assay Conditions

Gene/Protein Information For HTT (Source: Uniprot.org, NCBI)

Gene Name

HTT

Full Name

Huntingtin

Weight

347.603kDa

Superfamily

huntingtin family

Alternative Names

HD protein; huntingtin (Huntington disease); huntingtin; IT15HDHuntington disease protein HTT HD, IT15, LOMARS huntingtin huntingtin|huntington disease protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HTT, check out the HTT Infographic

HTT infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HTT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Huntington HTT Antibody

Question

We bought anti-Huntington antibody for WB on cervix carcinoma erythroleukemia a few months ago. I am using rat, and We are going to use the antibody for IF next. I was wanting to use examining cervix carcinoma erythroleukemia as well as muscle retina in our next experiment. Could give a recommendation on which antibody would work the best for IF?

M. Gonzalez

Verified customer

Asked: 2019-09-25

Answer

I have checked the website and datasheets of our anti-Huntington antibody and I see that A00134 has been tested on rat in both WB and IF. Thus A00134 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-09-25

Question

My boss were happy with the WB result of your anti-Huntington antibody. However we have seen positive staining in cervix carcinoma huntingtin using this antibody. Is that expected? Could you tell me where is HTT supposed to be expressed?

Z. Banerjee

Verified customer

Asked: 2017-09-21

Answer

From literature, cervix carcinoma does express HTT. Generally HTT expresses in huntingtin: cytoplasm, huntingtin, myristoylated n-terminal. Regarding which tissues have HTT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10332029, 11013077
Brain, Caudate nucleus, Frontal cortex, Muscle, and Retina, Pubmed ID: 7903579
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Retina, Pubmed ID: 8458085

Boster Scientific Support

Answered: 2017-09-21

Question

We have been able to see staining in rat brain. Do you have any suggestions? Is anti-Huntington antibody supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2017-09-06

Answer

According to literature brain does express HTT. According to Uniprot.org, HTT is expressed in cerebellum, retina, brain, brain, caudate nucleus, frontal cortex, muscle retina, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have HTT expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 10332029, 11013077
Brain, Caudate nucleus, Frontal cortex, Muscle, and Retina, Pubmed ID: 7903579
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Retina, Pubmed ID: 8458085

Boster Scientific Support

Answered: 2017-09-06

Question

We are currently using anti-Huntington antibody A00134 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?

O. Krishna

Verified customer

Asked: 2014-01-03

Answer

The anti-Huntington antibody (A00134) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-01-03

Order DetailsPrice
A00134

100 μg

$599

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