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SKU:PB9017
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-ICAM-1 Antibody
Description Rabbit IgG polyclonal antibody for Intercellular adhesion molecule 1(ICAM1) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-ICAM-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9017)
Replacement Item This antibody may replace the following items: sc-101702|sc-107|sc-135946|sc-1511|sc-1511-R|sc-18839|sc-18853|sc-18908|sc-31724|sc-31725|sc-366317|sc-390483|sc-53336|sc-71292|sc-7891|sc-8439 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human ICAM1 recombinant protein (Position: L214-P532). Human ICAM1 shares 52% and 48% amino acid (aa) sequences identity with mouse and rat ICAM1, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name ICAM1
Protein Name Intercellular adhesion molecule 1
Molecular Weight 57825 MW
Protein Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. .
Sequence Similarities Belongs to the immunoglobulin superfamily. ICAM family.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID P05362
Alternative Names Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1;
Research Areas |immunology|cell type markers|adhesion| cardiovascular|atherosclerosis|vascular inflammation|leukocyte recruitment|cell adhesion molecules| stem cells|hematopoietic progenitors|lymphoid|b lymphocytic lineage|t lymphocytic lineage| immunology|adaptive immunity|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Intercellular adhesion molecule 1

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). It is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.

Anti-ICAM-1 Antibody Images

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Anti-ICAM-1 Antibody
Anti-ICAM1 Picoband antibody, PB9017-1.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-ICAM-1 Antibody
Anti-ICAM1 Picoband antibody, PB9017-2.jpg
All lanes: Anti-ICAM1(PB9017) at 0.5ug/ml
Lane 1: HEPG2 Whole Cell Lysate at 40ug
Lane 2: K562 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: HT1080 Whole Cell Lysate at 40ug
Predicted bind size: 58KD
Observed bind size: 100KD
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Publications

Zhu W, Lv Q, Chen H, Wang Z, Zhong Q. J Huazhong Univ Sci Technolog Med Sci. 2011 Aug;31(4):441-5. Doi: 10.1007/S11596-011-0470-8. Epub 2011 Aug 7. Protective Effect And Mechanism Of Sodium Tanshinone Ii A Sulfonate On Microcirculatory Disturbance...
Wang Zz, Zhao Wj, Zhang Xs, Tian Xf, Wang Yz, Zhang F, Yuan Jc, Han Gz, Liu Kx, Yao Jh. World J Gastroenterol. 2007 Jan 28;13(4):564-71. Protection Of Veratrum Nigrum L. Var. Ussuriense Nakai Alkaloids Against Ischemia-Reperfusion Injury Of The Ra...
Zhang Y, Li J, Liu Cy, Zhou Xk, Qiu J, Zhang Yb, Huang Ny, Li Y, Chen Xj, Li Xl, Wang Ys, Yang Hs, Chen Xc, Kan B, Mao Yq, Deng Hx, Yang L, Wen Yj, Zhao X, Wei Yq. Dermatology. 2010 Aug;221(1):84-92. Doi: 10.1159/000314154. Epub 2010 Jun 18. A Nov...
Tian Xf, Yao Jh, Zhang Xs, Zheng Ss, Guo Xh, Wang Lm, Wang Zz, Liu Kx. Surg Today. 2010 Sep;40(9):858-65. Doi: 10.1007/S00595-009-4170-Y. Epub 2010 Aug 26. Protective Effect Of Carnosol On Lung Injury Induced By Intestinal Ischemia/Reperfusion.
Cheng Y, Zhang Y, Song H, Feng J. Neural Regen Res. 2012 Jun 15;7(17):1312-7. Doi: 10.3969/J.Issn.1673-5374.2012.17.005. Intercellular Adhesion Molecule-1 Expression In The Hippocampal Ca1 Region Of Hyperlipidemic Rats With Chronic Cerebral Ischemia.
Liu D, Zeng Bx, Zhang Sh, Yao Sl. Inflamm Res. 2005 Nov;54(11):464-70. Rosiglitazone, An Agonist Of Peroxisome Proliferator-Activated Receptor Gamma, Reduces Pulmonary Inflammatory Response In A Rat Model Of Endotoxemia.
Hang Ch, Shi Jx, Li Js, Li Wq, Yin Hx. World J Gastroenterol. 2005 Feb 28;11(8):1149-54. Up-Regulation Of Intestinal Nuclear Factor Kappa B And Intercellular Adhesion Molecule-1 Following Traumatic Brain Injury In Rats.
Hang Ch, Shi Jx, Tian J, Li Js, Wu W, Yin Hx. Brain Res. 2004 Nov 5;1026(1):23-32. Effect Of Systemic Lps Injection On Cortical Nf-Kappab Activity And Inflammatory Response Following Traumatic Brain Injury In Rats.
Wang J, Qiao L, Li Y, Yang G. Exp Mol Med. 2008 Dec 31;40(6):686-98. Ginsenoside Rb1 Attenuates Intestinal Ischemia-Reperfusion- Induced Liver Injury By Inhibiting Nf-Kappab Activation.
Wu J, Yang T, Wang C, Liu Q, Yao J, Sun H, Kaku T, Liu Kx. Biol Pharm Bull. 2008 Nov;31(11):2040-4. Laennec Protects Murine From Concanavalin A-Induced Liver Injury Through Inhibition Of Inflammatory Reactions And Hepatocyte Apoptosis.
Yao Jh, Zhang Xs, Zheng Ss, Li Yh, Wang Lm, Wang Zz, Chu L, Hu Xw, Liu Kx, Tian Xf. World J Gastroenterol. 2009 Jul 14;15(26):3240-5. Prophylaxis With Carnosol Attenuates Liver Injury Induced By Intestinal Ischemia/Reperfusion.
Xu Y, Feng L, Wang S, Zhu Q, Zheng Z, Xiang P, He B, Tang D. J Ethnopharmacol. 2011 Sep 1;137(1):359-70. Doi: 10.1016/J.Jep.2011.05.041. Epub 2011 Jun 6. Calycosin Protects Huvecs From Advanced Glycation End Products-Induced Macrophage Infiltration.
Wang W, Zhao F, Fang Y, Li X, Shen L, Cao T, Zhu H. Crit Care. 2013 Mar 11;17(2):R44. Doi: 10.1186/Cc12558. Glycyrrhizin Protects Against Porcine Endotoxemia Through Modulation Of Systemic Inflammatory Response.
Tu Ct, Han B, Liu Hc, Zhang Sc. Mol Cell Biochem. 2011 Dec;358(1-2):53-60. Doi: 10.1007/S11010-011-0920-4. Epub 2011 Jun 22. Curcumin Protects Mice Against Concanavalin A-Induced Hepatitis By Inhibiting Intrahepatic Intercellular Adhesion Molecule...
Ning C, Liu L, Lv G, Yang Y, Zhang Y, Yu R, Wang Y, Zhu J. Lipids Health Dis. 2015 Apr 18;14:31. Doi: 10.1186/S12944-015-0030-5. Lipid Metabolism And Inflammation Modulated By Vitamin D In Liver Of Diabetic Rats.
Wang Yq, Shen Y, Li F, Wang Ch, Zhang W. Cell Biochem Biophys. 2013;67(3):997-1004. Doi: 10.1007/S12013-013-9595-7. 2,3,4',5-Tetrahydroxystilbene-2-O-??-D-Glucoside Suppresses Expression Of Adhesion Molecules In Aortic Wall Of Dietary Atherosclerot...
Li Y, Chen Y, Zhang J, Zhu Jf, Liu Zj, Liang Sy, Sun K, Liao Wy, Gong Jp. Am J Surg. 2010 Jan;199(1):35-42. Doi: 10.1016/J.Amjsurg.2008.11.039. Protective Effect Of Glutamine-Enriched Early Enteral Nutrition On Intestinal Mucosal Barrier Injury Af...
Ji X, Gao J, Cai X, Lu W, Hu C, Wang Z, Cheng X, Gu Z, Wan G, Zhang S, Cao P. J Ethnopharmacol. 2011 Apr 12;134(3):624-9. Doi: 10.1016/J.Jep.2011.01.003. Epub 2011 Jan 8. Immunological Regulation Of Chinese Herb Guizhi Fuling Capsule On Rat Endome...
Cui Wy, Tian Ay, Bai T. Clin Exp Pharmacol Physiol. 2011 Nov;38(11):747-54. Doi: 10.1111/J.1440-1681.2011.05584.X. Protective Effects Of Propofol On Endotoxemia-Induced Acute Kidney Injury In Rats.
Huang Kf, Huang Xp, Xiao Gq, Yang Hy, Lin Js, Diao Y. Biomed Pharmacother. 2014 May;68(4):455-61. Doi: 10.1016/J.Biopha.2014.03.005. Epub 2014 Mar 18. Kallistatin, A Novel Anti-Angiogenesis Agent, Inhibits Angiogenesis Via Inhibition Of The Nf-??b ...
Zhang Yf, Yang X, Zhang Yj, Sun Yl, Zou Xl, Kong Qy, Dong Xq, Ye Xq, Yu Xq. Am J Nephrol. 2006;26(6):602-11. Epub 2006 Dec 20. Peroxisome Proliferator-Activated Receptor-Gamma Is Expressed By Rat Peritoneal Mesothelial Cells: Its Potential Role In...
Xiang M, Qian Zy, Zhou Ch, Liu J, Li Wn. J Ethnopharmacol. 2006 Aug 11;107(1):25-31. Epub 2006 Mar 10. Crocetin Inhibits Leukocyte Adherence To Vascular Endothelial Cells Induced By Ages.
Tian Xx, Wang Bl, Cao Yz, Zhong Yx, Tu Yy, Xiao Jb, He Qf, Zhai Ln. Chin J Integr Med. 2015 Mar;21(3):229-33. Doi: 10.1007/S11655-013-1513-8. Epub 2013 Oct 19. Comparison Of Protective Effects Of Safflor Injection And Extract Of Ginkgo Biloba On L...
Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induced...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to icam 1 antibody, icam-1 antibody, cd54 antibody, icam1 antibody