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SKU:PA1374
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-IGF-1 Antibody
SKU/Catalog Number PA1374
Description Rabbit IgG polyclonal antibody for Insulin-like growth factor I(IGF1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-IGF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1374)
Replacement Item This antibody may replace the following items: sc-1422|sc-7144|sc-74116|sc-9013 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human IGF-1(80-96 aa GSSSRRAPQTGIVDECC), different from the mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name IGF1
Protein Name Insulin-like growth factor I(IGF-I)
Molecular Weight 21841 MW
Protein Function The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. .
Sequence Similarities Belongs to the insulin family.
Subcellular Localization Secreted.
Uniprot ID P05019
Alternative Names Insulin-like growth factor I;IGF-I;Mechano growth factor;MGF;Somatomedin-C;IGF1;IBP1;
Research Areas |signal transduction|growth factors/hormones|insulin / insulin-like| cancer|growth factors|insulin and insulin-like| developmental biology|post embryonic development|aging|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Insulin-like growth factor I(IGF-I)

Insulin-like growth factor 1(IGF-1) also known as somatomedin C or mechano growth factor is a protein that in humans is encoded by the IGF1 gene. IGF-1 is a hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. A synthetic analog of IGF-1, mecasermin is used for the treatment of growth failure. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 has a molecular weight of 7649 daltons.Justice et al.(1990) placed the mouse IGF1 gene on chromosome 10.

Anti-IGF-1 Antibody Images

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Anti-IGF-1 Antibody
Anti-IGF-1 antibody, PA1374, Western blotting
WB: Recombinant Human IGF-1 Protein 2.5ng
Anti-IGF-1 Antibody
Anti-IGF-1 antibody, PA1374, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Publications

Li X, Zhang Z, Li Y, Zhao Y, Zhai W, Yang L, Kong D, Wu C, Chen Z, Teng CB. Sci Rep. 2017 Mar 23;7:45002. doi: 10.1038/srep45002. miR-18a counteracts AKT and ERK activation to inhibit the proliferation of pancreatic progenitor cells.
Fang-Ping Xu, PhD, MD; Yan-Hui Liu, BS; Xin-Lan Luo, BS; Fen Zhang,MS; Hai-Yu Zhou, PhD, MD; Yan Ge, PhD, MD1 Overexpression of SRC-3 promotes esophageal squamous cell carcinoma aggressiveness by enhancing cell growth and invasiveness
Tang H, Liao Y, Xu L, Zhang C, Liu Z, Deng Y, Jiang Z, Fu S, Chen Z, Zhou S. Int J Cancer. 2013 Nov 15;133(10):2473-82. Doi: 10.1002/Ijc.28262. Epub 2013 Jun 12. Estrogen And Insulin-Like Growth Factor 1 Synergistically Promote The Development Of ...
Huang P, Zhou Z, Wang H, Wei Q, Zhang L, Zhou X, Hutz Rj, Shi F. Int J Mol Med. 2012 Sep;30(3):650-8. Doi: 10.3892/Ijmm.2012.1041. Epub 2012 Jun 20. Effect Of The Igf-1/Pten/Akt/Foxo Signaling Pathway On The Development And Healing Of ...
Chai H, Guo L, Wang X, Fu Y, Guan J, Tan L, Ren L, Yang K. J Mater Sci Mater Med. 2011 Nov;22(11):2525-35. Doi: 10.1007/S10856-011-4427-Z. Epub 2011 Aug 26. Antibacterial Effect Of 317L Stainless Steel Contained Copper In Prevention Of Implant-Rel...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: How should I reconstitute my WB control (IGF-1 recombinant protein 100 ng/vial) for working with the mouse muscle tissue? I have tried a couple of different dilutions already. It appeared that much of the protein stayed lodged at the top of the gel and did not migrate (Evidence from the signal saturation at the top of the blot in the control lane).
A: We suggest you use 50 µL water to reconstitute with the 100 ng recombinant protein. Then mix 10 µL of this solution with 10 µL of 2X sample buffer so that the protein concentration is 1 ng/µl (i.e., 1 µg/mL).
Q: I did not see a band in my samples at 8 kDa. Instead, I saw a very well-defined clear band at about 12.5 kDa. The positive control had a signal at 10 kDa. Does the mouse IGF-1 run higher than the human IGF-1? I used a 15% acrylamide gel.
A; There might be problem that your pre-stained markers did not give the required precision on your gel. Note that the nominal molecular weight of mouse IGF-1 is different from that of human.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to igf1r antibody, igf 1 receptor antibody, igf 1r antibody, igfr antibody