Show Order Info
SKU:PA1498
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-IGFBP-3 Antibody
SKU/Catalog Number PA1498
Description Rabbit IgG polyclonal antibody for Insulin-like growth factor-binding protein 3(IGFBP3) detection. Tested with WB, IHC-P in Human.
Cite This Product Anti-IGFBP-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1498)
Replacement Item This antibody may replace the following items: sc-6004|sc-9028|sc-374365|sc-6003|sc-365936|sc-130786|sc-135947 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human IGFBP-3(182-198aa IKKGHAKDSQRYKVDYE).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name IGFBP3
Protein Name Insulin-like growth factor-binding protein 3(IBP-3/IGF-binding protein 3/IGFBP-3)
Molecular Weight 31674 MW
Protein Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. .
Tissue Specificity Expressed by most tissues. Present in plasma.
Sequence Similarities Contains 1 IGFBP N-terminal domain.
Subcellular Localization Secreted .
Uniprot ID P17936
Alternative Names Insulin-like growth factor-binding protein 3;IBP-3;IGF-binding protein 3;IGFBP-3;IGFBP3;IBP3;
Research Areas |cell biology|apoptosis|receptors|associated proteins| signal transduction|protein phosphorylation|tyrosine phosphatases| cardiovascular|angiogenesis|angiogenic factors|growth factors/hormones|insulin / insulin-like|atherosclerosis|diabetes associated| metabolism|types of disease|obesity| cancer|cell death|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Insulin-like growth factor-binding protein 3(IBP-3/IGF-binding protein 3/IGFBP-3)

IGFBP3, Insulin-like growth fator-binding protein 3, is a member of the insulin-like growth factor binding protein(IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. IGFBP3 is located on chromosome 7. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit(IGFALS) and either insulin-like growth factor(IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Anti-IGFBP-3 Antibody Images

Click the images to enlarge.

Anti-IGFBP-3 Antibody
Anti-IGFBP-3 antibody, PA1498, Western blotting
All lanes: Anti IGFBP-3 (PA1498) at 0.5ug/ml
Lane 1: 293T Whole Cell Lysate at 40ug
Lane 2: MCF- 7 Whole Cell Lysate at 40ug
Lane 3: A549 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Predicted bind size: 32KD
Observed bind size: 40KD
Anti-IGFBP-3 Antibody
Anti-IGFBP-3 antibody, PA1498, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Write a review for PA1498

Publications

Pu Xy, Zheng Xg, Zhang Y, Xiao Hj, Xu Zp, Liu Jm, Wang Hp, Wen Am, Zhou Xx, Wu Yl. J Sex Med. 2011 Aug;8(8):2181-90. Doi: 10.1111/J.1743-6109.2011.02318.X. Epub 2011 May 19. Higher Expression Of Mrna And Protein Of Insulin-Like Growth Factor Bindi...
Pu Xy, Wen Am, Liu Jm, Zheng Xg, Xiao Hj, Xu Zp, Wang Hp, Li D, Zhang Rl. J Urol. 2014 Sep;192(3):990-6. Doi: 10.1016/J.Juro.2014.02.042. Epub 2014 Feb 24. Shrna Constructs Targeting Igfbp-3 Alleviate Age Related Erectile Dysfunction In The Rat.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to igfbp 3 antibody, igfbp3 antibody, igfbp-3 antibody