Data & Images
|Product Name||Anti-IKK Gamma Antibody|
|Description||Rabbit IgG polyclonal antibody for NF-kappa-B essential modulator(IKBKG) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-IKK Gamma Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1551-1)|
|Replacement Item||This antibody may replace the following items: sc-166397|sc-166398|sc-166700|sc-293135|sc-31721|sc-52930|sc-56919|sc-71331|sc-8032|sc-8256|sc-8256-R|sc-8330 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human IKK gamma(97-114aa FQEARKLVERLGLEKLDL), different from the related mouse and rat sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||NF-kappa-B essential modulator(NEMO)|
|Molecular Weight||48198 MW|
|Protein Function||Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination. .|
|Tissue Specificity||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.|
|Sequence Similarities||Contains 1 C2HC-type zinc finger.|
|Subcellular Localization||Cytoplasm . Nucleus . Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress.|
|Alternative Names||NF-kappa-B essential modulator;NEMO;FIP-3;IkB kinase-associated protein 1;IKKAP1;Inhibitor of nuclear factor kappa-B kinase subunit gamma;I-kappa-B kinase subunit gamma;IKK-gamma;IKKG;IkB kinase subunit gamma;NF-kappa-B essential modifier;IKBKG;FIP3, NEMO;|
|Research Areas|||cell biology|apoptosis|intracellular|nfkb|ikk| signal transduction|protein phosphorylation|ser / thr kinases|other kinases|signaling pathway|nuclear signaling|nfkb pathway| epigenetics and nuclear signaling|nuclear signaling pathways| cardiovascular|atherosclerosis|vascular inflammation|inflammatory mediators| immunology|innate immunity|tlr signaling| cancer|cell death|apoptotic markers||
Background for NF-kappa-B essential modulator(NEMO)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-IKK Gamma Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-IKK Gamma Antibody Images
Click the images to enlarge.
All lanes: Anti IKBKG (PA1551-1) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Skeletal Muscle Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 48KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,