Anti-IL-17A Monoclonal Antibody

IL-17/IL-17A antibody

Boster Bio Anti-IL-17A Monoclonal Antibody (Catalog # M00421-1). Tested in WB applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00421-1
Size: 100ug
Reactive Species: Human
Host: Mouse
Application: WB

Product Name

Anti-IL-17A Monoclonal Antibody

View all IL-17/IL-17A Antibodies

SKU/Catalog Number

M00421-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-IL-17A Monoclonal Antibody (Catalog # M00421-1). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-IL-17A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00421-1)

Host

Mouse

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Monoclonal

Clone Number

Clone: 4H1524.1

Isotype

IgG2b

Immunogen

Anti-IL-17A (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

M00421-1 is reactive to IL17A in Human

Applications

M00421-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

17.504kDa

Background of IL-17/IL-17A

IL17-A (also known as Interleukin-17) is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17-A is the founding member of a group of cytokines called the IL17 family. IL17-A was originally identified as a transcript from a rodent T-cell hybridoma. To elicit its functions, IL17 binds to a type I cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000-1:50,000
Flow Cytometry: User optimized
WB: 1µg/mL

Validation Images & Assay Conditions

Gene/Protein Information For IL17A (Source: Uniprot.org, NCBI)

Gene Name

IL17A

Full Name

Interleukin-17A

Weight

17.504kDa

Superfamily

IL-17 family

Alternative Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL17A, check out the IL17A Infographic

IL17A infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL17A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00421-1 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.

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14 Customer Q&As for Anti-IL-17A Monoclonal Antibody

Question

Is there a BSA free version of anti-IL-17A Monoclonal antibody M00421-1 available?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL-17A Monoclonal antibody M00421-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-08

Question

Is a blocking peptide available for product anti-IL-17A Monoclonal antibody (M00421-1)?

Verified Customer

Verified customer

Asked: 2020-01-22

Answer

We do provide the blocking peptide for product anti-IL-17A Monoclonal antibody (M00421-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-22

Question

I was wanting to use your anti-IL-17A Monoclonal antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?

Verified Customer

Verified customer

Asked: 2019-12-02

Answer

It shows on the product datasheet, M00421-1 anti-IL-17A Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-02

Question

Does anti-IL-17A Monoclonal antibody M00421-1 work for WB with t-cell?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

According to the expression profile of t-cell, IL17A is highly expressed in t-cell. So, it is likely that anti-IL-17A Monoclonal antibody M00421-1 will work for WB with t-cell.

Boster Scientific Support

Answered: 2019-11-29

Question

My boss were content with the WB result of your anti-IL-17A Monoclonal antibody. However we have observed positive staining in palpebral conjunctiva secreted. using this antibody. Is that expected? Could you tell me where is IL17A supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

According to literature, palpebral conjunctiva does express IL17A. Generally IL17A expresses in secreted. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404

Boster Scientific Support

Answered: 2019-10-17

Question

Is this M00421-1 anti-IL-17A Monoclonal antibody reactive to the isotypes of IL17A?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

The immunogen of M00421-1 anti-IL-17A Monoclonal antibody is anti-IL-17A (MOUSE) Monoclonal antibody was produced in mouse by repeated immunizations with mature full length recombinant human IL-17A produced in E.coli followed by hybridoma development. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-09

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-IL-17A Monoclonal antibody M00421-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-07-01

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-01

Question

We are currently using anti-IL-17A Monoclonal antibody M00421-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

The anti-IL-17A Monoclonal antibody (M00421-1) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-27

Question

Would M00421-1 anti-IL-17A Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-05-22

Answer

You can see on the product datasheet, M00421-1 anti-IL-17A Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-05-22

Question

We have seen staining in human palpebral conjunctiva. Are there any suggestions? Is anti-IL-17A Monoclonal antibody supposed to stain palpebral conjunctiva positively?

Verified Customer

Verified customer

Asked: 2019-04-02

Answer

Based on literature palpebral conjunctiva does express IL17A. Based on Uniprot.org, IL17A is expressed in palpebral conjunctiva, t-cell, among other tissues. Regarding which tissues have IL17A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 7499828, 14574404

Boster Scientific Support

Answered: 2019-04-02

Question

Here is the WB image, lot number and protocol we used for t-cell using anti-IL-17A Monoclonal antibody M00421-1. Please let me know if you require anything else.

O. Jones

Verified customer

Asked: 2019-02-21

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-02-21

Question

I see that the anti-IL-17A Monoclonal antibody M00421-1 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-23

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-23

Question

My question regarding product M00421-1, anti-IL-17A Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Johnson

Verified customer

Asked: 2013-05-08

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00421-1 anti-IL-17A Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2013-05-08

Question

My lab would like to test anti-IL-17A Monoclonal antibody M00421-1 on human t-cell for research purposes, then I may be interested in using anti-IL-17A Monoclonal antibody M00421-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

R. Zhang

Verified customer

Asked: 2013-01-18

Answer

The products we sell, including anti-IL-17A Monoclonal antibody M00421-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-01-18

Order DetailsPrice
M00421-1

100 μg

$612

Free Secondary Antibody

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M00421-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$612.00

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